HYALURONIC-ACID CAPSULE IS A VIRULENCE FACTOR FOR MUCOID GROUP-A STREPTOCOCCI
HYALURONIC-ACID CAPSULE IS A VIRULENCE FACTOR FOR MUCOID GROUP-A STREPTOCOCCI
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DOI：
10.1073/pnas.88.19.8317
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发表时间：
1991-10-01
影响因子：
11.1
通讯作者：
DICESARE, TJ
中科院分区：
文献类型：
--
作者：
WESSELS, MR;MOSES, AE;DICESARE, TJ
A组链球菌的粘液型菌株与最近急性风湿热的爆发有关。这些菌株的粘液样菌落形态是大量产生由透明质酸组成的荚膜多糖的结果。为了研究透明质酸囊在致病性中的作用，我们通过转座子诱变从A组链球菌的粘液菌株中获得了一个无囊突变体。突变株中M蛋白表达没有改变。粘液样野生型菌株在新鲜人血中生长，并且在体外对吞噬杀伤具有抗性。相比之下，无囊突变体不能在新鲜人血中生长，并且对体外吞噬细胞杀伤敏感。胶囊的损失与小鼠中微生物毒力降低100倍有关。我们得出结论，透明质酸胶囊保护粘液A组链球菌从吞噬作用，并具有重要的作用，毒力。
Mucoid strains of group A Streptococcus have been associated with recent outbreaks of acute rheumatic fever. The mucoid colony morphology of these strains is a result of abundant production of capsular polysaccharide, which is composed of hyaluronic acid. To study the role of the hyaluronic acid capsule in virulence, we derived an acapsular mutant from a mucoid strain of group A Streptococcus by transposon mutagenesis. M protein expression was not altered in the mutant strain. The mucoid wild-type strain grew in fresh human blood and was resistant to phagocytic killing in vitro. In contrast, the acapsular mutant failed to grow in fresh human blood and was sensitive to phagocytic killing in vitro. Loss of capsule was associated with a 100-fold reduction in virulence of the organisms in mice. We conclude that the hyaluronic acid capsule protects mucoid group A streptococci from phagocytosis and has an important role in virulence.