Upregulation of P2X2 and P2X3 receptors in rats with hyperalgesia induced by heroin withdrawal
Upregulation of P2X2 and P2X3 receptors in rats with hyperalgesia induced by heroin withdrawal
复制标题
海洛因戒断引起的痛觉过敏大鼠中 P2X2 和 P2X3 受体的上调。
DOI：
10.1097/wnr.0000000000001018
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发表时间：
2018-05-23
期刊：
影响因子：
1.7
通讯作者：
Li, Chaoying
中科院分区：
文献类型：
--
作者：
Leng, Changlong;Chen, Lin;Li, Chaoying
阿片类药物突然停药所致的药物依赖和戒断综合征仍是临床治疗慢性疼痛和阿片类药物成瘾的严重障碍。阿片类药物戒断的关键症状之一是痛觉过敏。阿片类药物戒断所致痛敏的机制尚不清楚。P2X2和P2X3受体是P2X受体亚单位的成员，是多种伤害性刺激的整合因子，在慢性神经病理性和炎症性疼痛的伤害性感受转导中发挥重要作用。P2X2和P2X3受体拮抗过程抑制炎性痛觉过敏，涉及脊髓阿片系统。然而，P2X受体在阿片类药物戒断所致痛敏中的作用很少被讨论。为探讨P2X2和P2X3受体在阿片类药物引起的痛敏反应中的作用，采用海洛因自身给药大鼠，在戒断海洛因8d后，采用爪部缩足实验评价大鼠的热痛觉和机械痛觉阈值。用免疫荧光法检测背根神经节中P2X2、P2X3受体的表达。结果表明，戒断8天后，海洛因给药大鼠出现热痛敏和机械性痛敏。同时，背根神经节中P2X2和P2X3受体的表达增加。这些结果提示，P2X2和P2X3受体的上调可能在海洛因戒断所致的痛敏中起部分作用。版权所有(C)2018 Wolters Kluwer Health，Inc.保留所有权利。
Drug dependence and withdrawal syndrome induced by abrupt cessation of opioid administration remain a severe obstacle in the clinical treatment of chronic pain and opioid drug addiction. One of the key symptoms during opioid withdrawal is hyperalgesia. The mechanism of opioid withdrawal-induced hyperalgesia remains unclear. P2X2 and P2X3 receptors, members of P2X receptor subunits, act as the integrator of multiple forms of noxious stimuli and play an important role in nociception transduction of chronic neuropathic and inflammatory pain. The process of P2X2 and P2X3 receptor antagonism inhibits inflammatory hyperalgesia, involving the spinal opioid system. However, the role of P2X receptors involved in opioid withdrawal-induced hyperalgesia has seldom been discussed. To explore the role of P2X2 and P2X3 receptors in the opioid-induced hyperalgesia, heroin self-administration rats were adopted, and the thermal and mechanical nociceptive thresholds were evaluated using the paw withdrawal test after abstinence from heroin for 8 days. In addition, the expressions of P2X2 and P2X3 receptors in dorsal root ganglia were analyzed by immunofluorescence. The results showed that after 8 days of abstinence, heroin self-administration rats showed thermal hyperalgesia and mechanical allodynia. Meanwhile, the expressions of the P2X2 and P2X3 receptors in dorsal root ganglia were increased. These results suggest that upregulation of P2X2 and P2X3 receptors might partially play a role in heroin withdrawal-induced hyperalgesia. Copyright (C) 2018 Wolters Kluwer Health, Inc. All rights reserved.