SO, a protein involved in hyphal fusion in Neurospora crassa, localizes to septal plugs
SO, a protein involved in hyphal fusion in Neurospora crassa, localizes to septal plugs
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DOI：
10.1128/ec.00268-06
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发表时间：
2007-01-01
期刊：
影响因子：
--
通讯作者：
Glass, N. Louise
中科院分区：
文献类型：
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作者：
Fleissner, Andre;Glass, N. Louise
丝状子囊菌真菌的菌落通常以多核合胞体的形式生长。虽然这种合胞组织具有发育优势，但也有菌丝局部损伤造成大面积损伤的风险。受损菌丝的细胞质损失是由一种叫做Woronin体的过氧化物酶体来源的细胞器快速有效地封闭菌丝交叉壁或间隔的中心孔所限制的。隔塞的形成也与发育有关，并导致蜂群某些部分的分离。与发育过程或菌丝老化有关的间隔塞通常是由密封物质的积累引起的。在这里，我们报道了在粗神经孢子虫中，一种叫做SO （SOFT）的蛋白质是菌丝融合和菌落发育所必需的，它位于间隔塞上。作为对损伤的反应，SO以不依赖于Woronin体的方式在间隔塞处积累。然而，Woronin体的存在影响了SO在间隔孔积聚的速度。我们确定SO有助于鼻中隔堵塞，但不是必不可少的。在菌丝老化和异核不相容（het）位点的遗传差异介导的程序性细胞死亡期间形成的间隔塞中也观察到SO积累。
The colony of a filamentous ascomycete fungus typically grows as a multinucleate syncytium. While this syncytial organization has developmental advantages, it bears the risk of extensive damage caused by local injury of hyphae. Loss of cytoplasm in injured hyphae is restricted by the fast and efficient sealing of the central pores of hyphal crosswalls, or septa, by a peroxisome-derived organelle called the Woronin body. The formation of septal plugs is also associated with development and leads to separation of certain parts of the colony. Septal plugs associated with developmental processes or aging hyphae typically occur by the accumulation of sealing material. Here we report that in Neurospora crassa, a protein necessary for hyphal fusion and proper colony development called SO (SOFT) localizes to septal plugs. In response to injury, SO accumulates at the septal plug in a Woronin body-independent manner. However, the presence of the Woronin body affects the speed of accumulation of SO at the septal pore. We determined that SO contributes to, but is not essential for, septal plugging. SO accumulation was also observed at septal plugs formed during hyphal aging and during programmed cell death mediated by genetic differences at heterokaryon incompatibility (het) loci.