Deterministic Lateral Displacement as a Means to Enrich Large Cells for Tissue Engineering
Deterministic Lateral Displacement as a Means to Enrich Large Cells for Tissue Engineering
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DOI：
10.1021/ac9018395
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发表时间：
2009-11-01
影响因子：
7.4
通讯作者：
Murthy, Shashi K.
中科院分区：
文献类型：
--
作者：
Green, James V.;Radisic, Milica;Murthy, Shashi K.
在组织工程领域，需要从异质悬浮液中富集或分离选定的细胞类型。用于这种分离的最先进技术包括预镀和基于筛网的方法，这些方法的纯度和产量范围有限。在这里，我们提出了一种确定性横向位移（DLD）微流体装置，它能够将大上皮细胞（直径17.3 +/- 2.7）与小成纤维细胞（直径13.7 +/- 3.0 μ m）分离，作为一种潜在的替代方法。所检测的混合悬浮液旨在代表消化后的大鼠心脏组织的含量，其中包含相同比例的心肌细胞（17.0 +/- 4.0 μ m直径）和非心肌细胞群体（12.0 +/- 3.0 μ m直径）。高纯度分离（bbb97 %）的大细胞类型实现了90%的收率，快速和单道工艺。这项工作的意义在于认识到DLD设计原理可以应用于大细胞的微流控富集，达到40 μ m直径的水平。
The enrichment or isolation of selected cell types from heterogeneous suspensions is required in the area of tissue engineering. State of the art techniques utilized for this separation include preplating and sieve-based approaches that have limited ranges of purity and variable yield. Here, we present a deterministic lateral displacement (DLD) microfluidic device that is capable of separating large epithelial cells (17.3 +/- 2.7 in diameter) from smaller fibroblast cells (13.7 +/- 3.0 mu m in diameter) as a potential alternative approach. The mixed suspension examined is intended to represent the content of digested rat cardiac tissue, which contains equal proportions of cardiomyocyte (17.0 +/- 4.0 mu m diameter) and nonmyocyte populations (12.0 +/- 3.0 mu m diameter). High purity separation (>97%) of the larger cell type is achieved with 90% yield in a rapid and single-pass process. The significance of this work lies in the recognition that DLD design principles can be applied for the microfluidic enrichment of large cells, up to the 40 mu m diameter level examined in this work.