Applications of Imaging Spectroscopy in Molecular Biology: I. Screening Photosynthetic Bacteria
Applications of Imaging Spectroscopy in Molecular Biology: I. Screening Photosynthetic Bacteria
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成像光谱在分子生物学中的应用：一、筛选光合细菌
DOI：
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发表时间：
1988
期刊：
影响因子：
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通讯作者：
D. Youvan
中科院分区：
文献类型：
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作者：
Mary M. Yang;D. Youvan
我们开发了一种成像分光光度计，它结合了摄像机的空间分辨特性和分光光度计的波长分辨特性。该仪器特别适用于筛选光合细菌，因为可以通过光合色素的发色特性同时分析数百个菌落。增强荧光突变体（具有缺陷的光合反应中心）可以使用硅靶相机实时成像。通过成像光谱法进一步促进突变体筛选，其中混合在特定波长下获得的图像以产生菌落的伪图像。然后可以使用数字图像处理技术来突出菌落的区别光谱特征。成像光谱学的其他领域的分子生物学的未来应用进行了讨论。
We have developed an imaging spectrophotometer that combines the spatial-resolving properties of a video camera with the wavelength-resolving properties of a spectrophotometer. This instrument is especially useful for screening photosynthetic bacteria, since hundreds of colonies may be analyzed simultaneously through the chromophoric properties of the photosynthetic pigments. Enhanced fluorescence mutants (possessing defective photosynthetic reaction centers) can be imaged in realtime using a silicon target camera. Mutant screening is further facilitated through imaging spectroscopy, wherein images obtained at specific wavelengths are mixed to generate pseudo images of the colonies. Digital image processing techniques can then be used to highlight distinguishing spectroscopic features of the colonies. Future applications of imaging spectroscopy to other areas of molecular biology are discussed.