Genetic diversity and variation of saponin contents in Panax notoginseng roots from a single farm
Genetic diversity and variation of saponin contents in Panax notoginseng roots from a single farm
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DOI：
10.1021/jf051248g
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发表时间：
2005-11-02
影响因子：
6.1
通讯作者：
Hong, Y
中科院分区：
文献类型：
--
作者：
Hong, DYQ;Lau, AJ;Hong, Y
三七，三七的根。陈芳华，在中药中应用广泛。据报道，其主要成分皂苷具有多种药理活性。为了验证在同一地点种植和收获的同一种草药在化学和基因组成上是一致的这一普遍假设，进行了化学分析和DNA指纹鉴定。采用高效薄层色谱法和高效液相色谱法对同一农场随机抽取的17根3年生的根进行了6种皂苷的分析。随着人参皂苷Rd/Rg1、Re/Rg1和Rb1/Rg1含量的变化，5根人参皂苷的化学特征也不同。同样的样品，连同一些1岁和2岁的样品，也进行了荧光扩增片段长度多态性（AFLP）分析，并对其内部转录间隔区2 （ITS 2）进行了测序。荧光AFLP分析发现其多态性比ITS 2序列高得多，并且在被测群体中显示了遗传多样性的明确证据。结果表明，三七各根间皂苷含量存在遗传多样性和变异。我们认为遗传多样性影响了六种皂苷的含量。在中国和新加坡市场的三七根样品中也发现了皂苷含量的差异和遗传多样性。由于不同的皂苷含量可能会影响治疗效果，将遗传图谱与化学图谱结合使用将有助于确保三七根质量的更大一致性。种群内的遗传和化学多样性也为培育具有更理想化学成分的新品种提供了机会。
Radix notoginseng, the root of Panax notoginseng (Burk.) F. H. Chen, has been widely used in traditional Chinese medicine. Its main components, saponins, have been reported to have many pharmacological activities. To test the general assumption that herbs of a single species planted and harvested from a single location are uniform in chemical and genetic makeup, chemical analysis and DNA fingerprinting were carried out. High-performance TLC together with HPLC analysis were used to analyze 17 randomly sampled 3-year-old roots from a single farm for the presence of six saponins. Five roots showed distinct chemical profiles with changed ratios of ginsenosides Rd/Rg1, Re/Rg1, or Rb1/Rg1. The same samples, together with some 1- and 2-year-old samples, were also subjected to fluorescent amplified fragment length polymorphism (AFLP) analysis, and their internal transcribed spacer 2 (ITS 2) regions were sequenced. Fluorescent AFLP analysis was found to be much more polymorphic than the ITS 2 sequence and showed clear evidence of genetic diversity within the tested population. In conclusion, genetic diversity and variation of saponin contents between individual P. notoginseng roots have been detected. We suggest that genetic diversity affects the contents of the six saponins. The saponin contents variation and genetic diversity were also found among P. notoginseng root samples collected from China and Singapore markets. Since variable saponin contents may affect therapeutic efficacy, combining the use of genetic profiling with chemical profiling will help ensure greater uniformity in the quality of P. notoginseng roots. The genetic and chemical diversity within a population also provides the opportunity for breeding new cultivars with more desirable chemical constituents.