The novel early region 3 protein E3/49K is specifically expressed by adenoviruses of subgenus D: Implications for epidemic keratoconjunctivitis and adenovirus evolution
The novel early region 3 protein E3/49K is specifically expressed by adenoviruses of subgenus D: Implications for epidemic keratoconjunctivitis and adenovirus evolution
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DOI：
10.1006/viro.2002.1404
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发表时间：
2002-04-25
期刊：
影响因子：
3.7
通讯作者：
Burgert, HG
中科院分区：
文献类型：
--
作者：
Blusch, JH;Deryckere, F;Burgert, HG
腺病毒的早期转录单位3(E3)编码免疫调节功能。我们先前在Ad19a的E3区描述了一个新的49K基因，它是D亚属的一个与AD8和AD37相似的基因，可引起流行性角膜结膜炎(EKC)。有趣的是，据报道，49K在Ad9和AD17中不存在，而其他D亚属的ADS不会导致EKC。因此，我们调查了49K是否选择性地在导致EKC的ADS中表达。利用特异的DNA探针，我们在所测试的所有D亚属ADS中检测到49K同源基因。此外，49K特异性抗体在感染所有D亚型血清型的细胞中识别高分子量蛋白质，无论它们是否有能力导致EKC。对几个49K基因的测序显示，与EKC相关的Ad株的同源性很高，但没有一个明显的特征可识别。因此，E3/49K是D亚属特有的E3蛋白，其表达与引起EKC的表型无关，因此可能与该亚属常见的疾病有关。有趣的是，Ad19a和AD37的49K序列是相同的。为了估计这两种病毒之间的序列一致性程度，我们最初对正确的ITR和六邻体进行了测序。分析表明，Ad19a的右侧ITR序列与AD37完全相同，而六邻体序列与Ad19p相似。这表明，识别区域要大得多，Ad19a是由AD37与Ad19p样腺病毒重组而产生的。进一步的测序定位了DNA结合蛋白内的交叉。因此，Ad19a包含一个大的序列块(类似于13kb)，从100K基因到右侧的ITR，与AD37相同。根据引起EKC的Ad菌株的时间现象，讨论了这些发现的含义。(C)2002年埃尔塞维尔科学公司(美国)。
The early transcription unit 3 (E3) of adenoviruses (Ads) encodes immunomodulatory functions. We previously described a novel gene of 49K within the E3 region of Ad19a, an Ad of subgenus D that is similar to Ad8 and Ad37 causes epidemic keratoconjunctivitis (EKC). Interestingly, 49K was reported not to be present in Ad9 and Ad17, other subgenus D Ads not causing EKC Therefore, we investigated whether 49K is selectively expressed in EKC-causing Ads. Using specific DNA probes, we detect 49K-homologous genes in all subgenus D Ads tested. Moreover, 49K-specific antibodies recognize a high molecular weight protein in cells infected with all subgenus D serotypes irrespective of their ability to cause EKC. Sequencing of several 49K genes reveals a high homology without a distinct feature recognizable for those of EKC-associated Ad strains. Thus, E3/49K is a subgenus D specific E3 protein whose expression does not correlate with the EKC-causing phenotype and thus may rather be implicated in illnesses commonly caused by this subgenus. Interestingly, the 49K sequences of Ad 19a and Ad37 are identical. To estimate the extent of the sequence identity between these two viruses, we initially sequenced the right ITR and the hexon. This analysis revealed that the right ITR of Ad19a is identical to Ad37, while the hexon sequence is Ad19p-like. This suggested that the region of identity is much larger and that Ad19a arose by recombination of Ad37 with an Ad19p-like Ad. Further sequencing mapped the crossover within the DNA binding protein. Thus, Ad19a contains a large sequence block (similar to13 kb), from the 100K gene to the right ITR, identical to Ad37. The implications of these findings in light of the temporal appearance of the EKC-causing Ad strains are discussed. (C) 2002 Elsevier Science (USA).