Non-vascular smooth muscle cells in the human choroid: distribution, development and further characterization
Non-vascular smooth muscle cells in the human choroid: distribution, development and further characterization
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DOI：
10.1111/j.1469-7580.2005.00460.x
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发表时间：
2005-10-01
影响因子：
2.4
通讯作者：
May, CA
中科院分区：
文献类型：
--
作者：
May, CA
为了进一步表征人眼脉络膜中的非血管平滑肌细胞（NVSMC），我们进行了广泛的形态学研究，包括成人眼睛中NVSMC的三维分布及其发育过程中的外观。42名人类供体（孕13周至89岁）的眼睛的整块和切片的脉络膜和巩膜被针对平滑肌肌动蛋白和其他平滑肌细胞标记物的抗体染色。根据其形态定位，在成人眼中可区分出三组非瓣膜性小细胞瘤：(a)非瓣膜性小细胞瘤在脉络膜上、巩膜内、睫状后动脉及神经入口周围呈半圆形排列；(b)平行于眼后段纤毛后动脉入口点与旋涡静脉出口点之间血管的NVSMC；(c)脉络膜上致密的斑块状NVSMC排列，覆盖在中央凹区域。最后一组表现出最明显的个体间差异。在发育过程中，第一个在妊娠第20周观察到的NVSMC属于b组。一个完整的NVSMC网络首次在一个6岁的供眼中观察到。所有三组在所有定位中均呈光滑蛋白、钙调蛋白和钙钙蛋白阳性。NVSMC在脉络膜和脉络膜上的分布不同，可能反映了其功能的不同方面。所有细胞在组织化学上表现为真正的可收缩性。
To characterize further non-vascular smooth muscle cells (NVSMC) in the choroid of the human eye, extensive morphological studies were performed including a three-dimensional distribution of NVSMC in the adult human eye and their appearance during development. Whole mounts and sections through the choroid and sclera of eyes of 42 human donors (between the 13th week of gestation and 89 years of age) were stained with antibodies against smooth muscle actin and other markers for smooth muscle cells. On the basis of their morphological localization, three groups of NVSMC could be distinguished in the adult eyes: (a) a semicircular arrangement of NVSMC in the suprachoroid and inner sclera, around the entry of posterior ciliary arteries and nerves; (b) NVSMC parallel to the vessels in the posterior eye segment between the point of entry of the posterior ciliary arteries and the point of exit of the vortex veins; and (c) a dense plaque-like arrangement of NVSMC in the suprachoroid, overlying the foveal region. The last of these groups showed most pronounced interindividual differences. During development, the first NVSMC to be observed at the 20th week of gestation belonged to group b. A complete NVSMC network was first observed in a 6-year-old donor eye. All three groups stained positive for smoothelin, caldesmon and calponin in all localizations. The NVSMC show a distinct distribution that might reflect different aspects of their function in the choroid and suprachoroid. All cells could be histochemically characterized as truly contractile.