Electron spin resonance spectroscopic studies of free radical generation and tissue specific catalase gene expression in Spodoptera litura (Fab.) larvae treated with the mycotoxin, destruxin
Electron spin resonance spectroscopic studies of free radical generation and tissue specific catalase gene expression in Spodoptera litura (Fab.) larvae treated with the mycotoxin, destruxin
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DOI：
10.1016/j.pestbp.2010.01.002
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发表时间：
2010-07-01
影响因子：
4.7
通讯作者：
Bhatnagar, Raj K.
中科院分区：
文献类型：
--
作者：
Sree, K. Sowjanya;Sachdev, Bindiya;Bhatnagar, Raj K.
从Metarhizium anisopliae (Metch.) 中回收的Detruxin 具有杀虫作用。在本研究中，在部分纯化的destruxin处理的斜纹夜蛾(Fab.)幼虫中观察到超氧自由基和NADPH依赖的超氧自由基水平以剂量和时间依赖性方式显着增加。经处理的斜纹夜蛾幼虫中羟基自由基的ESR谱显示自由基水平随时间和剂量依赖性增加。研究了处理1、24和48小时的斜纹夜蛾幼虫全身过氧化氢酶基因的转录本丰度。以时间方式进行了同样的研究，即中肠、脂肪体、唾液腺、马氏小管和斜纹夜蛾血细胞。在作为解毒器官的脂肪体中观察到过氧化氢酶转录水平最高，而在暴露于毒素时容易受到最大损害的唾液腺中观察到最低过氧化氢酶转录水平。 (C) 2010 Elsevier Inc. 保留所有权利。
Destruxin recovered from Metarhizium anisopliae (Metch.) is insecticidal in nature. In the present study, a marked increase in the levels of superoxide radical and NADPH dependent superoxide radical in the partially purified destruxin treated larvae of Spodoptera litura (Fab.) was observed in a dose and time dependent manner. The ESR spectrum of the hydroxyl radical in the treated S. litura larvae depicted a time and dose dependent increase in the free radical level. Transcript abundance of catalase gene in S. litura larvae treated for 1, 24 and 48 h was investigated in the whole body. The same was studied in a temporal fashion i.e. midgut, fat body, salivary gland, Malphigian tubule and haemocytes of S. litura. The maximum catalase transcript level was observed in the fat body which is a detoxifying organ and the least in salivary gland which was prone to maximum damage upon exposure to the toxin. (C) 2010 Elsevier Inc. All rights reserved.