PASSIVE-IMMUNIZATION AGAINST CACHECTIN TUMOR NECROSIS FACTOR PROTECTS MICE FROM LETHAL EFFECT OF ENDOTOXIN
PASSIVE-IMMUNIZATION AGAINST CACHECTIN TUMOR NECROSIS FACTOR PROTECTS MICE FROM LETHAL EFFECT OF ENDOTOXIN
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DOI：
10.1126/science.3895437
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发表时间：
1985-01-01
期刊：
影响因子：
56.9
通讯作者：
CERAMI, AC
中科院分区：
文献类型：
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作者：
BEUTLER, B;MILSARK, IW;CERAMI, AC
制备了一种高度特异性的兔抗鼠恶病质素/肿瘤坏死因子（TNF）多克隆抗血清。当用该抗血清或纯化的免疫球蛋白对BALB/c小鼠进行被动免疫时，它们可免受大肠杆菌产生的内毒素脂多糖的致死作用。预防效果呈剂量依赖性，且在注射内毒素之前给予抗血清时效果最佳。抗恶病质素/TNF的抗血清并不能减轻内毒素处理动物的发热反应，而且极高剂量的内毒素可克服其保护作用。用50微升特异性抗血清预处理的小鼠的内毒素半数致死量（LD50）约为给予非免疫血清的对照组LD50的2.5倍。恶病质素/TNF可能是内毒素致死作用的主要介质之一。
A highly specific polyclonal rabbit antiserum directed against murine cachectin/tumor necrosis factor (TNF) was prepared. When BALB/c mice were passively immunized with the antiserum or with purified immune globulin, they were protected against the lethal effect of the endotoxin lipopolysaccharide produced by Escherichia coli. The prophylactic effect was dose-dependent and was most effective when the antiserum was administered prior to the injection of the endotoxin. Antiserum to cachectin/TNF did not mitigate the febrile response of endotoxin-treated animals and very high doses of endotoxin could overcome the protective effect. The LD50 of endotoxin in mice pretreated with 50 .mu.l of the specific antiserum was .apprx. 2.5 times greater the LD50 for controls given nonimmune serum. Chectin/TNF maybe one of the principal mediators of the lethal effect of endotoxin.