The relationship of growth and adenylate cyclase activity in cultured thyroid cells: separate bioeffects of thyrotropin.
The relationship of growth and adenylate cyclase activity in cultured thyroid cells: separate bioeffects of thyrotropin.
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培养的甲状腺细胞中生长和腺苷酸环化酶活性的关系：促甲状腺素的单独生物效应。
DOI：
10.1210/endo-112-1-71
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发表时间：
1983
期刊：
影响因子：
4.8
通讯作者：
F. Ambesi
中科院分区：
文献类型：
--
作者：
W. Valente;P. Vitti;L. Kohn;M. Brandi;C. Rotella;R. Toccafondi;D. Tramontano;S. Aloj;F. Ambesi
利用TSH依赖的功能大鼠甲状腺细胞克隆系，比较了TSH对cAMP生成和细胞生长的影响，通过细胞数量和[14C]胸腺嘧啶掺入来测量。通过测量细胞数量，从培养基中提取TSH停止了细胞的生长。在无TSH培养基中24 h后，1 × 10-11 M纯TSH急性刺激可使cAMP水平增加40倍；当从培养系统中提取TSH的时间延长10天以上时，这种反应增加到80倍以上。与这些对cAMP水平的急性影响相反，[14C]胸腺嘧啶掺入的增加需要20倍高的TSH浓度，并在TSH停药10天后减弱到无反应水平。从培养基中去除TSH 10天后，重新添加TSH仅在2天滞后期才能使细胞恢复正常生长速率。在滞后性方面，TSH对细胞数量的影响与其对胸腺嘧啶掺入的影响是平行的，而不是cAMP水平；2-cAMP at…
Using a clonal line of TSH-dependent functional rat thyroid cells, the effects of TSH on cAMP production and cell growth, as measured by cell number and [14C]thymidine incorporation, were compared. The withdrawal of TSH from the medium stopped the growth of cells, as measured by cell number. After 24 h in TSH-free medium, an acute challenge with 1 × 10-11 M pure TSH caused a 40-fold increase in the cAMP level; this response increased to greater than 80-fold as TSH withdrawal from the culture system was prolonged over 10 days. In contrast to these acute effects on cAMP levels, an increase in [14C]thymidine incorporation required 20-fold higher TSH concentrations and waned to an unresponsive level after 10 days of TSH withdrawal. After withdrawal of TSH from the medium for 10 days, readdition of TSH could return cell growth to normal rates only after a 2-day lag period. In terms of the lag, the TSH effect on cell number was paralleled by its effect on thymidine incorporation, not cAMP levels; (Bu)2-cAMP at ...