Rapid Active Zone Remodeling during Synaptic Plasticity
Rapid Active Zone Remodeling during Synaptic Plasticity
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DOI：
10.1523/jneurosci.6698-10.2011
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发表时间：
2011-04-20
影响因子：
5.3
通讯作者：
Eilers, Jens
中科院分区：
文献类型：
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作者：
Weyhersmueller, Annika;Hallermann, Stefan;Eilers, Jens
突触如何改变突触可塑性期间释放的神经递质的量？虽然释放一般是深入研究，其在可塑性的决定因素仍然知之甚少。作为一个模型的塑料加强突触释放，我们在这里使用的突触前稳态补偿干扰突触后谷氨酸受体在果蝇神经肌肉接头。结合短期可塑性分析，累积EPSC分析，波动分析，和量子短期可塑性建模，我们发现在突触前强化的释放准备囊泡的数量增加。高分辨率光学显微镜显示的活性区蛋白Bruchpilot和扩大的突触前细胞基质结构的量增加。此外，活动区的这些功能和结构改变不仅在终身后观察到，而且在突触前强化几分钟后就已经观察到了。我们的研究结果表明，突触前可塑性可以诱导活动区重塑，在几分钟内调节释放准备囊泡的数量。
How can synapses change the amount of neurotransmitter released during synaptic plasticity? Although release in general is intensely investigated, its determinants during plasticity are still poorly understood. As a model for plastic strengthening of synaptic release, we here use the well-established presynaptic homeostatic compensation during interference with postsynaptic glutamate receptors at the Drosophila neuromuscular junction. Combining short-term plasticity analysis, cumulative EPSC analysis, fluctuation analysis, and quantal short-term plasticity modeling, we found an increase in the number of release-ready vesicles during presynaptic strengthening. High-resolution light microscopy revealed an increase in the amount of the active zone protein Bruchpilot and an enlargement of the presynaptic cytomatrix structure. Furthermore, these functional and structural alterations of the active zone were not only observed after lifelong but already after minutes of presynaptic strengthening. Our results demonstrate that presynaptic plasticity can induce active zone remodeling, which regulates the number of release-ready vesicles within minutes.