Daam1 regulates the endocytosis of EphB during the convergent extension of the zebrafish notochord
Daam1 regulates the endocytosis of EphB during the convergent extension of the zebrafish notochord
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DOI：
10.1073/pnas.0608946104
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发表时间：
2007-04-17
影响因子：
11.1
通讯作者：
Ogura, Toshihiko
中科院分区：
文献类型：
--
作者：
Kida, Yasuyuki S.;Sato, Takayuki;Ogura, Toshihiko
细胞的会聚伸展运动是控制组织器官有组织形态发生的基本过程之一。然而，连接非经典Writ途径和CE运动的分子事件还不清楚。我们发现，亚细胞定位Daaml，一个重要组成部分的非经典的书面信号，脊索形成过程中动态变化。在早期阶段，Daaml与EphB受体和Disheveled 2复合。该复合物以动力蛋白依赖性的方式被掺入内吞囊泡中，从而导致EphB从细胞表面去除，随后切换细胞膜。在下一步中，Daaml与肌动蛋白细胞骨架共定位以诱导细胞的形态延伸。我们阐明了脊索细胞CE运动的分子机制，Daaml作为一个动态的协调员的包被和细胞骨架重塑。
Convergent extension (CE) movement of cells is one of the fundamental processes that control the organized morphogenesis of tissues and organs. The molecular events connecting the noncanonical Writ pathway and CE movement, however, are not well understood. We show that subcellular localization of Daaml, an essential component of noncanonical Writ signaling, changes dynamically during notochord formation. In the early phases, Daaml complexes with EphB receptors and Disheveled 2. This complex is incorporated into endocytic vesicles in a dynamin-dependent manner, thereby resulting in the removal of EphB from the cell surface with subsequent switching of cell adhesiveness. In the next step, Daaml colocalizes with the actin cytoskeleton to induce morphological extension of cells. We elucidate the molecular mechanism underlying the CE movement of notochord cells with Daaml as a dynamic coordinator of enclocytosis and cytoskeletal remodeling.