Identification of a Gene Sharing a Promoter and Peroxisome Proliferator-Response Elements With Acyl-CoA Oxidase Gene
Identification of a Gene Sharing a Promoter and Peroxisome Proliferator-Response Elements With Acyl-CoA Oxidase Gene
复制标题
与酰基辅酶A氧化酶基因共享启动子和过氧化物酶体增殖物反应元件的基因的鉴定
作者：
M. Akter;M. Razzaque;Liu Yang;Toshio Fumoto;K. Motojima;Tomohiro Yamaguchi;F. Hirose;T. Osumi
许多哺乳动物基因在基因组上聚集，因此同一簇中的基因可以通过共同的调控元件进行调控。我们以前确实表明perilipin/PEX 11 α基因对分别通过一个共同的结合位点被PPARγ和PPARα组织选择性地反式激活。在本研究中，我们确定了一个基因，命名为GSPA，邻近的一个典型的过氧化物酶体增殖物激活受体的目标，酰基辅酶A氧化酶（AOX）基因。过氧化物酶体增殖剂Wy 14，643在野生型小鼠的肝脏中诱导GSPA表达，但在PPARα缺失小鼠的肝脏中不诱导GSPA表达。GSPA和AOX共享启动子和两个过氧化物酶体增殖反应元件。在心脏和骨骼肌以及3 T3-L1细胞中也发现了GSPA mRNA。GSPA编码在3 T3-L1细胞中富集的161个氨基酸的蛋白质。甚至其他基因对也可能通过共同的序列元件进行调节，相反，每个基因如何在簇中适当地进行调节将是有趣的。
Many mammalian genes are clustered on the genomes, and hence the genes in the same cluster can be regulated through a common regulatory element. We indeed showed previously that the perilipin/PEX11α gene pair is transactivated tissue-selectively by PPARγ and PPARα, respectively, through a common binding site. In the present study, we identified a gene, named GSPA, neighboring a canonical PPAR target, acyl-CoA oxidase (AOX) gene. GSPA expression was induced by a peroxisome proliferator, Wy14,643, in the liver of wild-type mice, but not PPARα-null mice. GSPA and AOX share the promoter and two peroxisome proliferator-response elements. GSPA mRNA was also found in the heart and skeletal muscle, as well as 3T3-L1 cells. GSPA encodes a protein of 161 amino acids that is enriched in 3T3-L1 cells. Even other gene pairs might be regulated through common sequence elements, and conversely it would be interesting how each gene is aptly regulated in clusters.