Proteomic analysis of the enterocyte brush border
Proteomic analysis of the enterocyte brush border
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DOI：
10.1152/ajpgi.00005.2011
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发表时间：
2011-05-01
影响因子：
4.5
通讯作者：
Tyska, Matthew J.
中科院分区：
文献类型：
--
作者：
McConnell, Russell E.;Benesh, Andrew E.;Tyska, Matthew J.
首页--期刊主要分类--期刊细介绍--期刊题录与文摘--期刊详细文摘内容肠细胞刷状缘的蛋白质组学分析。Am J生理学胃肠病肝生理学300：G914-G926,2011。2011年2月17日首次出版；doi：10.1152/ajpgi.00005.2011。-肠上皮细胞顶端的刷状边界区域是肠道吸收养分的主要部位，也是与腔内微生物相互作用的主要表面。因为刷子边缘位于这样一个关键的生理界面上，我们开始使用鸟枪质谱仪创建一个驻留在这个结构域中的蛋白质的全面列表。由此得到的蛋白质组包含646种具有不同功能的蛋白质。除了预期的营养加工和运输成分的收集外，我们还确定了有望在肌动蛋白动力学、膜弯曲和细胞外黏附调节中发挥作用的分子。这些结果为未来旨在确定支持刷子边缘组装和功能的分子机制的研究提供了基础。
McConnell RE, Benesh AE, Mao S, Tabb DL, Tyska MJ. Proteomic analysis of the enterocyte brush border. Am J Physiol Gastrointest Liver Physiol 300: G914-G926, 2011. First published February 17, 2011; doi:10.1152/ajpgi.00005.2011.-The brush border domain at the apex of intestinal epithelial cells is the primary site of nutrient absorption in the intestinal tract and the primary surface of interaction with microbes that reside in the lumen. Because the brush border is positioned at such a critical physiological interface, we set out to create a comprehensive list of the proteins that reside in this domain using shotgun mass spectrometry. The resulting proteome contains 646 proteins with diverse functions. In addition to the expected collection of nutrient processing and transport components, we also identified molecules expected to function in the regulation of actin dynamics, membrane bending, and extracellular adhesion. These results provide a foundation for future studies aimed at defining the molecular mechanisms underpinning brush border assembly and function.