Antimicrobial activity of ellagic acid against Helicobacter pylori isolates from India and during infections in mice
Antimicrobial activity of ellagic acid against Helicobacter pylori isolates from India and during infections in mice
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DOI：
10.1093/jac/dky079
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发表时间：
2018-06-01
影响因子：
5.2
通讯作者：
Mukhopadhyay, Asish K.
中科院分区：
文献类型：
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作者：
De, Ronita;Sarkar, Avijit;Mukhopadhyay, Asish K.
目的：由于抗生素耐药性的上升，一种廉价的，基于饮食的治疗幽门螺杆菌感染将是极大的兴趣。本研究旨在评估鞣花酸对临床H。pylori菌株的耐药性的抗生素用于治疗，并观察形态结构后，用鞣花酸处理。鞣花酸对H. pylori感染的小鼠（C57 BL/6）感染模型，一个标准的近交系小鼠常用于实验感染，也assessed.Methods：共55株进行了筛选。琼脂稀释法用于测定分离株对供试化合物的敏感性。用透射电镜观察鞣花酸处理后的细胞形态。鞣花酸对H.结果：鞣花酸对55株H. pylori SS 1感染小鼠的生长均有抑制作用。幽门螺杆菌菌株测试。鞣花酸的最低抑菌浓度为5 ~ 30 mg/L，显示其体外杀菌特性。鞣花酸也表现出抗H。pylori在体内模型中根除该有机体的功效，以及H.结论：本研究为鞣花酸预防和治疗幽门螺杆菌引起的胃粘膜损伤奠定了基础。pylori感染，因此，鞣花酸可以被认为是一种有前途的抗幽门螺杆菌的抗菌剂。幽门相关性胃十二指肠疾病。
Objectives: Because of the rise in antimicrobial resistance, an inexpensive, diet-based treatment against Helicobacter pylori infection would be of great interest. The present study was performed to assess the in vitro effects of ellagic acid against clinical H. pylori strains that were resistant to antibiotics used for therapy and also to observe the morphological structure following treatment with ellagic acid. The effectiveness of ellagic acid in eradicating H. pylori infection in a murine (C57BL/6) infection model, one of the standard inbred mouse lines often used for experimental infection, was also assessed.Methods: A total of 55 strains were screened. The agar dilution method was used to determine the susceptibility of isolates to test compounds. Transmission electron microscopy was used to observe the morphology following treatment with ellagic acid. The antibacterial activity of ellagic acid in an H. pylori SS1-infected mouse model and its effect on gastric mucosal injury were determined by histology and PCR.Results: Ellagic acid inhibited the growth of all 55 of the H. pylori strains tested. The MIC of ellagic acid ranged from 5 to 30 mg/L, showing its bactericidal properties in vitro. Ellagic acid also demonstrated anti-H. pylori efficacy in eradication of this organismin an in vivo model, as well as restitution and repair of H. pylori-induced gastricmucosal damage.Conclusions: The present study paves the way for the preventive and therapeutic use of ellagic acid against H. pylori infection and, thus, ellagic acid can be considered a promising antibacterial agent against H. pylori-associated gastroduodenal diseases in humans.