Jun-Mediated Changes in Cell Adhesion Contribute to Mouse Embryonic Stem Cell Exit from Ground State Pluripotency.
Jun-Mediated Changes in Cell Adhesion Contribute to Mouse Embryonic Stem Cell Exit from Ground State Pluripotency.
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DOI：
10.1002/stem.2294
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发表时间：
2016-05
期刊：
影响因子：
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通讯作者：
Sharrocks AD
中科院分区：
文献类型：
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作者：
Veluscek G;Li Y;Yang SH;Sharrocks AD
胚胎干细胞（ESC）能够产生任何类型的体细胞。关于ESC多能性是如何维持的，我们知道得很多，但关于分化是如何促进的，我们知道得相对较少。细胞命运决定受信号传导和转录网络之间的相互作用调节。最近的研究表明，转录因子Jun的过表达或下调可以影响ESC的命运。在这里，我们重点关注了Jun在小鼠胚胎干细胞从基态多能性退出和早期分化开始中的作用。对分化中的ESC的转录组学分析表明，当ESC退出多能基态时，Jun需要上调与细胞粘附相关的基因的程序。这些Jun调控的基因中的几个被证明是有效粘附所必需的。重要的是，这种粘附对于ESC从基态多能性的及时调节退出和早期分化事件的开始是必需的。干细胞2016;34：1213-1224
Embryonic stem cells (ESC) are able to give rise to any somatic cell type. A lot is known about how ESC pluripotency is maintained, but comparatively less is known about how differentiation is promoted. Cell fate decisions are regulated by interactions between signaling and transcriptional networks. Recent studies have shown that the overexpression or downregulation of the transcription factor Jun can affect the ESC fate. Here we have focussed on the role of the Jun in the exit of mouse ESCs from ground state pluripotency and the onset of early differentiation. Transcriptomic analysis of differentiating ESCs reveals that Jun is required to upregulate a programme of genes associated with cell adhesion as ESCs exit the pluripotent ground state. Several of these Jun‐regulated genes are shown to be required for efficient adhesion. Importantly this adhesion is required for the timely regulated exit of ESCs from ground state pluripotency and the onset of early differentiation events. Stem Cells 2016;34:1213–1224