Kininogen-kallikrein-kinin system in plasma and saliva of patients with Sjögren's syndrome.
Kininogen-kallikrein-kinin system in plasma and saliva of patients with Sjögren's syndrome.
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发表时间：
1998-12
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通讯作者：
C. C. Hernández-C.;E. Donadi;M. L. Reis
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作者：
C. C. Hernández-C.;E. Donadi;M. L. Reis
目的探讨干燥综合征（SS）患者血浆和唾液中激肽原-激肽释放酶-激肽系统（KKKS）的变化。方法选择20例37-75岁的SS患者，其中原发性SS（SS 1）7例，继发于类风湿关节炎的SS（SS 2）13例，并与20例健康人对照。通过ELISA评估总激肽原以及高分子量激肽原和低分子量激肽原（分别为HKg和LKg）水平。通过对选择性显色底物的酶活性测定血浆和组织激肽释放酶的活性。结果：患者血浆总激肽原、HKg和LKg水平及血浆激肽释放酶活性与对照组无显著差异。血浆中的组织激肽释放酶样活性和唾液中的活性组织激肽释放酶在SS患者中显著增加，而患者的总唾液组织激肽释放酶活性与对照组无显著差异。患者唾液中的蛋白质浓度显著增加，并且观察到唾液蛋白水平与活性组织激肽释放酶之间呈正相关。结论SS患者唾液中组织激肽释放酶总量与活性的比较表明，组织激肽释放酶大部分以活性形式存在。此外，我们观察到血浆中组织激肽释放酶样活性的伴随增加。这些结果表明SS患者血浆和唾液中KKKS的活化增加。
OBJECTIVE To evaluate variables of the kininogen-kallikrein-kinin system (KKKS) simultaneously in plasma and saliva of patients with Sjögren's syndrome (SS). METHODS We studied a group of 20 female patients with SS aged 37-75 years, 7 with primary SS (SS1) and 13 with SS secondary to rheumatoid arthritis (SS2), and 20 healthy individuals. Total kininogen and high and low molecular weight kininogen (HKg and LKg, respectively) levels were evaluated by ELISA. The activity of plasma and tissue kallikreins was determined by enzyme activity on selective chromogenic substrates. RESULTS The plasma levels of total kininogen, HKg, and LKg, and the activity of plasma kallikrein observed in patients were not significantly different from controls. The tissue kallikrein-like activity in plasma and the active tissue kallikrein in saliva were significantly increased in patients with SS, whereas the total salivary tissue kallikrein activity in patients was not significantly different from controls. The concentration of protein in the saliva of patients was significantly increased, and a positive correlation between salivary protein levels and the active tissue kallikrein was observed. CONCLUSION Comparisons between the total and the active tissue kallikrein in saliva of patients with SS showed that most of the tissue kallikrein was in its active form. In addition, we observed a concomitant increase of the tissue kallikrein-like activity in plasma. These results suggest increased activation of the KKKS in plasma and saliva of patients with SS.