FVIII production by human lung microvascular endothelial cells
FVIII production by human lung microvascular endothelial cells
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DOI：
10.1182/blood-2005-11-4571
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发表时间：
2006-07-15
期刊：
影响因子：
20.3
通讯作者：
Kirkpatrick, Charles James
中科院分区：
文献类型：
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作者：
Jacquemin, Marc;Neyrinck, Arne;Kirkpatrick, Charles James
虽然肝外因子VIII （FVIII）的合成足以止血，但肝外产生部位尚未明确。因此，我们研究了人类肺部产生FVIII的能力。拒绝移植的心脏供者的肺在孤立的再灌注模型中灌注和通气2小时。4个实验中有3个实验记录灌注介质中FVIII和血管性血液病因子（VWF）的渐进性积累。相比之下，因子V、纤维蛋白原和免疫球蛋白G （IgG）水平在灌注期间保持不变，表明FVIII和VWF的积累不是由于细胞间介质扩散到血管系统。纯化的人肺微血管内皮细胞在体外至少2次传代过程中产生FVIII。总之，这些数据确定了人类肺内皮细胞是FVIII的产生位点。
While extrahepatic factor VIII (FVIII) synthesis suffices for hemostasis, the extrahepatic production sites are not well defined. We therefore investigated the ability of the human lungs to produce FVIII. Lungs from heart-beating donors who were declined for transplantation were perfused and ventilated in an isolated reperfusion model for 2 hours. A progressive accumulation of FVIII and von Willebrand factor (VWF) was recorded in the perfusion medium in 3 of 4 experiments. By contrast, factor V, fibrinogen, and immunoglobulin G (IgG) levels remained constant during the perfusion period, indicating that the accumulation of FVIII and VWF was not due to diffusion from the intercellular medium into the vascular system. Purified human lung microvascular endothelial cells produced FVIII during at least 2 passages in vitro. Altogether, these data identify the lung endothelial cells as a FVIII production site in humans.