Graphene foam/hydrogel scaffolds for regeneration of peripheral nerve using ADSCs in a diabetic mouse model
Graphene foam/hydrogel scaffolds for regeneration of peripheral nerve using ADSCs in a diabetic mouse model
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DOI：
10.1007/s12274-021-3961-3
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发表时间：
2021-12
期刊：
影响因子：
9.9
通讯作者：
Qun Huang;Yuting Cai;Xinrui Yang;Weimin Li;Hongji Pu;Zhenjing Liu;Hongwei Liu;Mohsen Tamtaji
中科院分区：
文献类型：
--
作者：
Qun Huang;Yuting Cai;Xinrui Yang;Weimin Li;Hongji Pu;Zhenjing Liu;Hongwei Liu;Mohsen Tamtaji
周围神经损伤（PNI）的功能恢复不理想，而糖尿病（DM）及其相关并发症进一步削弱了糖尿病PNI（DPNI）的恢复。脂肪源性干细胞（adipose-derivedstemcells，ADSCs）来源丰富，具有良好的分化能力和旁分泌能力，是治疗DPNI的理想候选细胞。结果表明，脂肪干细胞能显著促进雪旺细胞和内皮细胞的增殖、迁移和管腔形成。ADSCs通过调节Nrf 2/HO-1、NF-κB和PI 3 K/AKT/mTOR信号通路，在减轻氧化应激、炎症反应、调节细胞代谢、生长、存活、增殖、血管生成、雪旺细胞分化和髓鞘形成等方面发挥多种作用。在目前的研究中，开发了新型石墨烯泡沫（GF）/水凝胶基支架来递送ADSCs用于治疗DPNI。GF/水凝胶支架具有良好的机械强度、合适的多孔网络结构、上级导电性和良好的生物相容性，体外实验结果显示GF/水凝胶支架能明显促进雪旺细胞的增殖。此外，体内实验表明，ADSCs负载GF/水凝胶支架显着促进DPNI的恢复，并抑制靶肌肉的萎缩，从而为DPNI患者提供了一种新的和有吸引力的治疗方法。
The functional recovery of peripheral nerve injury (PNI) is unsatisfactory, whereas diabetes mellitus (DM) and its related complications further attenuate the restoration of diabetic PNI (DPNI). Adipose-derived stem cells (ADSCs) are promising candidates for treatment of DPNI due to their abundant source, excellent differentiation and paracrine ability. Our results showed that ADSCs remarkably enhanced the proliferation and migration of Schwann cells and endothelial cells, and tube formation. Mechanistically, ADSCs could regulate Nrf2/HO-1, NF-κB and PI3K/AKT/mTOR signaling pathways, showing multiple functions in reducing oxidative stress and inflammation, and regulating cell metabolism, growth, survival, proliferation, angiogenesis, differentiation of Schwann cell and myelin formation. In current study, novel graphene foam (GF)/hydrogel-based scaffold was developed to deliver ADSCs for treatment of DPNI. GF/hydrogel scaffold exhibited excellent mechanical strength, suitable porous network, superior electrical conductivity, and good biocompatibility.In vitroresults revealed that GF/hydrogel scaffold could obviously accelerate proliferation of Schwann cells. Moreover,in vivoexperiments demonstrated that ADSCs-loaded GF/hydrogel scaffold significantly promoted the recovery of DPNI and inhibited the atrophy of targeted muscles, thus providing a novel and attractive therapeutic approach for DPNI patients.