FLUORESCENCE-POLARIZATION MEASUREMENTS ON NORMAL AND MUTANT HUMAN-SKIN FIBROBLASTS
FLUORESCENCE-POLARIZATION MEASUREMENTS ON NORMAL AND MUTANT HUMAN-SKIN FIBROBLASTS
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DOI：
10.1016/0003-9861(78)90113-3
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发表时间：
1978-01-01
影响因子：
3.9
通讯作者：
CHIAPPELLI, F
中科院分区：
文献类型：
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作者：
HAGGERTY, DF;KALRA, VK;CHIAPPELLI, F
在暴露于1，6-二苯基-1，3，5-己三烯后，测量完整的二倍体皮肤成纤维细胞中的荧光偏振，以估计细胞膜的脂质相的流动性。来自4例纯合子家族性高胆固醇血症患者的细胞的膜脂质比从其他13个人的正常和非相关的突变基因型的细胞中获得的流体状态时，所有的培养物生长在培养基上与天然血清。在这些条件下具有增加的流动性的膜脂质的唯一其它细胞类型是来自患有Lesch-Nyhan综合征的患者的1个成纤维细胞系。对Lesch-Nyhan成纤维细胞的另外2个非血缘系的检查显示，异常高水平的脂质流动性并不是该基因型细胞膜的共同特性。在含有脂质耗尽血清（几乎没有脂蛋白结合的固醇）的培养基中孵育的培养物引起正常细胞膜的流动性的可逆增加，其值与高胆固醇血症细胞的值相似，但对后者的膜脂质流动性没有影响。暴露于胆固醇不结合脂蛋白在无血清培养基中的文化导致在normo和hypercholesterolemic成纤维细胞的膜的脂质流动性下降。
Measurements of fluorescence polarization in intact diploid skin fibroblasts after exposure to 1,6-diphenyl-1,3,5-hexatriene were used to estimate the fluidity of the lipid phase(s) of cellular membranes. The membrane lipids of cells derived from 4 patients with homozygous familial hypercholesterolemia were in a more fluid state than those of cells obtained from 13 other individuals of normal and nonrelated mutant genotypes when all cultures were grown on medium with native serum. The only other cell type having membrane lipids of increased fluidity under these conditions was 1 fibroblast line derived from a patient with the Lesch-Nyhan syndrome. Examination of 2 additional nonconsanguinous lines of Lesch-Nyhan fibroblasts revealed that an abnormally high level of lipid fluidity was not a common property of the membranes of cells of this genotype. Incubation of cultures in medium containing lipid-depleted serum (virtually devoid of lipoprotein-bound sterol) caused a reversible increase in the fluidity of the membranes of normal cells to values similar to those of the hypercholesterolemic cells, but had no effect on the membrane-lipid fluidity of the latter. Exposure of cultures to cholesterol not bound to lipoprotein in serum-free medium resulted in a decrease in the lipid fluidity of the membranes of both normo- and hypercholesterolemic fibroblasts.