Nonlinear optical collagen cross-linking and mechanical stiffening: a possible photodynamic therapeutic approach to treating corneal ectasia
Nonlinear optical collagen cross-linking and mechanical stiffening: a possible photodynamic therapeutic approach to treating corneal ectasia
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DOI：
10.1117/1.jbo.18.3.038003
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发表时间：
2013-03-01
影响因子：
3.5
通讯作者：
Jester, James V.
中科院分区：
文献类型：
--
作者：
Chai, Dongyul;Juhasz, Tibor;Jester, James V.
在这项研究中，我们测试的假设，非线性光学（NLO）的多光子光激活核黄素使用聚焦飞秒（FS）激光可以用来诱导交联（CXL）和机械交联胶原作为一种潜在的临床治疗圆锥角膜和角膜扩张的治疗。使用调谐至760 nm并设置为100 mW（NLO CXL I）或150 mW（NLO CXL II）激光功率的FS激光器使核黄素浸泡的压缩胶原水凝胶交联。使用0.75 NA物镜透镜将FS脉冲聚焦到水凝胶中，并对水凝胶进行三维扫描。通过压痕测试测量水凝胶刚度表明，与基线值相比，NLO CXL I和II后计算的弹性模量（E）值显著增加两倍以上（P < 0.05）。此外，NLO CXL与单光子UVA CXL之间没有显著差异（P > 0.05）。该数据表明，NLO CXL在机械硬化胶原蛋白方面具有与传统UVA CXL相当的效果，并且可以提供一种安全有效的方法来将CXL定位在角膜内的不同区域和深度。c作者。由SPIE在知识共享署名3.0未移植许可下发布。
In this study we test the hypothesis that nonlinear optical (NLO) multiphoton photoactivation of riboflavin using a focused femtosecond (FS) laser light can be used to induce cross-linking (CXL) and mechanically stiffen collagen as a potential clinical therapy for the treatment of keratoconus and corneal ectasia. Riboflavin-soaked, compressed collagen hydrogels are cross-linked using a FS laser tuned to 760 nm and set to either 100 mW (NLO CXL I) or 150 mW (NLO CXL II) of laser power. FS pulses are focused into the hydrogel using a 0.75 NA objective lens, and the hydrogel is three-dimensionally scanned. Measurement of hydrogel stiffness by indentation testing show that the calculated elastic modulus (E) values are significantly increased over twofold following NLO CXL I and II compared with baseline values (P < 0.05). Additionally, no significant differences are detected between NLO CXL and single photon, UVA CXL (P > 0.05). This data suggests that NLO CXL has a comparable effect to conventional UVA CXL in mechanically stiffening collagen and may provide a safe and effective approach to localize CXL at different regions and depths within the cornea. c The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License.