Update on techniques for cryopreservation of human spermatozoa.
Update on techniques for cryopreservation of human spermatozoa.
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DOI：
10.4103/aja20229
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发表时间：
2022-11
影响因子：
2.9
通讯作者：
Zhu WB
中科院分区：
文献类型：
--
作者：
Huang C;Tang YL;Hu JL;Zhou WJ;Huang ZH;Luo XF;Li Z;Zhu WB
在20世纪60年代，精子冷冻保存被开发为一种保存生育能力的方法。目前，人类精子冷冻保存技术已广泛应用于辅助生殖领域。然而，尽管精子冷冻生物学已经取得了显著的成就，但冷冻保存后恢复活精子的最佳方法仍然难以捉摸。解冻后精子质量会受到冷冻保护剂、冰形成、储存条件和冷冻过程中渗透压的影响。本文综述了近年来在冷冻保存技术、冷冻保护剂、冷冻和解冻方法以及冷冻保存精子的新适应症等方面的研究进展。
In the 1960s, sperm cryopreservation was developed as a method to preserve fertility. Currently, techniques for the cryopreservation of human spermatozoa have been widely used in assisted reproduction. However, although sperm cryobiology has made notable achievements, the optimal method for the recovery of viable spermatozoa after cryopreservation remains elusive. Postthawing sperm quality can be affected by cryoprotectants, ice formation, storage conditions, and osmotic stress during the freezing process. This review discusses recent advances in different cryopreservation techniques, cryoprotectants, and freezing and thawing methods during cryopreservation and new indications for the use of cryopreserved spermatozoa.
影响因子：
3.1
作者：
Desai, Nina;Goldberg, Jeffrey;Austin, Cynthia;Sabanegh, Edmund;Falcone, Tommaso
通讯作者：
Falcone, Tommaso