MODIFICATION OF ENZYMATIC ISOTOPIC ASSAY OF HISTAMINE AND ITS APPLICATION TO MEASUREMENT OF HISTAMINE IN TISSUES, SERUM AND URINE
MODIFICATION OF ENZYMATIC ISOTOPIC ASSAY OF HISTAMINE AND ITS APPLICATION TO MEASUREMENT OF HISTAMINE IN TISSUES, SERUM AND URINE
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DOI：
10.1016/0009-8981(72)90419-6
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发表时间：
1972-01-01
影响因子：
5
通讯作者：
JACOBSEN, S
中科院分区：
文献类型：
--
作者：
BEAVEN, MA;HORAKOVA, Z;JACOBSEN, S
本文介绍了一种改良的组织匀浆、血清和尿液中组胺的酶同位素测定法。该试验基于Snyderet等人1的试验，其中组胺通过与组胺-N-甲基转移酶和S-腺苷甲硫氨酸-[14 C]甲基孵育转化为[14 C]甲基组胺，但进行了修改，以定量[14 C]-甲基组胺的形成和回收，并减少其他含14 C物质的提取。改进后的方法提高了对0.1 ng组胺的灵敏度，并可同时测定多达80至100个样品。该测定法特别适用于测定低组胺水平组织中的组胺含量，其中Shoreet等人2的荧光测定法给出了虚假的高结果。酶测定法还具有这样的优点，即组胺可以直接在组织匀浆、血浆、血清和尿液中测量，而不需要预先提取组胺。在人肿瘤和正常组织中检测到各种量的组胺，但在人血清中未检测到组胺（低于0.5 ng/ml）。正常人尿组胺排泄量平均为16 ± 14（± SD）μg组胺/24 h。
A modified enzymatic isotopic assay for histamine in tissue homogenates, serum and urine is described. The assay is based upon that of Snyderet al.1in which histamine is converted to [14C]methylhistamine by incubation with histamine-N-methyltransferase andS-adenosylmethionine-[14C]methyl but is modified to make the formation and recovery of [14C]-methylhistamine quantitative and to reduce the extraction of other14C-containing material. The modifications enhance the sensitivity of the assay to 0.1 ng histamine and permit the simultaneous measurement of as many as 80 to 100 samples. The assay is particularly useful for determining the histamine content in tissues with low histamine levels where the fluorometric assay of Shoreet al.2gives spuriously high results. The enzymatic assay also has the advantage that histamine can be measured directly in tissue homogenates, plasma, serum, and urine without the need for prior extraction of the histamine. Various amounts of histamine were detected in human tumors and normal tissues but none (less than 0.5 ng/ml) in human serum. Normal human urinary histamine excretion averaged 16 ± 14 (± SD) μg histamine/24 h.