Tuning the Multifunctionality of Iron Oxide Nanoparticles Using Self-Assembled Mixed Lipid Layers
Tuning the Multifunctionality of Iron Oxide Nanoparticles Using Self-Assembled Mixed Lipid Layers
复制标题
使用自组装混合脂质层调节氧化铁纳米颗粒的多功能性
DOI：
10.1021/acs.bioconjchem.7b00483
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发表时间：
2017
影响因子：
4.7
通讯作者：
Bothun, Geoffrey D.
中科院分区：
文献类型：
--
作者：
Preiss, Matthew R.;Cournoyer, Eily;Paquin, Karissa L.;Vuono, Elizabeth A.;Belanger, Kayla;Walsh, Edward;Howlett, Niall G.;Bothun, Geoffrey D.
We present an approach to tuning the multifunctionality of iron oxide nanoparticles (IONs) using mixed self-assembled monolayers of cationic lipid and anionic polyethylene glycol (PEG) lipid. By forming stable, monodispersed lipid-coated IONs (L-IONs) through a solvent-exchange technique, we were able to demonstrate the relationship between surface charge, the magnetic transverse relaxivity (r2from T2-weighted images), and the binding capacity of small interfering ribonucleic acids (siRNAs) as a function of the cationic-to-anionic (PEG) lipid ratio. These properties were controlled by the cationic charge and the PEG conformation; relaxivity and siRNA binding could be varied in the mushroom and brush regimes but not at high brush densities. In vitro results combining cell viability, uptake, and transfection efficiency using HeLa cells suggest that the functional physicochemical and biological properties of L-IONs may be best achieved using catanionic lipid coatings near equimolar ratios of cationic to anionic PEG–lipids.
