Dynamic responses of Bacteroides thetaiotaomicron during growth on glycan mixtures.
Dynamic responses of Bacteroides thetaiotaomicron during growth on glycan mixtures.
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DOI：
10.1111/mmi.12228
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发表时间：
2013-06
影响因子：
3.6
通讯作者：
Martens EC
中科院分区：
文献类型：
--
作者：
Rogers TE;Pudlo NA;Koropatkin NM;Bell JS;Moya Balasch M;Jasker K;Martens EC
拟杆菌（Bacteroides thetaiotaomicron, Bt）是一种人类结肠共生体，可降解许多不同的复杂碳水化合物（聚糖），其特性和数量可能会在每餐之间频繁而突然地变化。为了了解这种生物对动态生长条件的反应，我们用一系列不同的聚糖混合物挑战它，并测量了涉及聚糖分解代谢的反应。我们的研究结果表明，单个Bt细胞可以同时对多种聚糖作出反应，并且对新的聚糖的反应非常迅速。替代碳水化合物的存在不会改变反应动力学，但会降低一些聚糖利用基因的表达以及细胞对低浓度聚糖的敏感性。在含有12种不同聚糖的混合物中生长表明，Bt优先使用某些聚糖而不是其他聚糖。这种代谢层次不会因先前暴露于低优先级聚糖而改变，因为在培养后期重新引入高优先级底物会重新启动参与降解低优先级底物的基因的抑制。至少有一些碳水化合物优先效应发生在单糖识别水平上。我们的研究结果深入了解了细菌聚糖多能手如何在动态聚糖环境中改变其反应，并为解释体内相关代谢行为提供了必要的知识。
Bacteroides thetaiotaomicron (Bt) is a human colonic symbiont that degrades many different complex carbohydrates (glycans), the identities and amounts of which are likely to change frequently and abruptly from meal-to-meal. To understand how this organism reacts to dynamic growth conditions, we challenged it with a series of different glycan mixtures and measured responses involved in glycan catabolism. Our results demonstrate that individual Bt cells can simultaneously respond to multiple glycans and that responses to new glycans are extremely rapid. The presence of alternative carbohydrates does not alter response kinetics, but reduces expression of some glycan utilization genes as well as the cell’s sensitivity to glycans that are present in lower concentration. Growth in a mixture containing twelve different glycans revealed that Bt preferentially uses some before others. This metabolic hierarchy is not changed by prior exposure to lower priority glycans because re-introducing high priority substrates late in culture re-initiates repression of genes involved in degrading those with lower priority. At least some carbohydrate prioritization effects occur at the level of monosaccharide recognition. Our results provide insight into how a bacterial glycan generalist modifies its responses in dynamic glycan environments and provide essential knowledge to interpret related metabolic behavior in vivo.
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影响因子：
30.3
作者：
Martens EC;Chiang HC;Gordon JI
通讯作者：
Gordon JI
影响因子：
64.5
作者：
Sonnenburg ED;Zheng H;Joglekar P;Higginbottom SK;Firbank SJ;Bolam DN;Sonnenburg JL
通讯作者：
Sonnenburg JL
影响因子：
17.1
作者：
Turnbaugh PJ;Ridaura VK;Faith JJ;Rey FE;Knight R;Gordon JI
通讯作者：
Gordon JI
DOI：
10.1074/jbc.m109.008094
发表时间：
2009-07-03
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Martens EC;Roth R;Heuser JE;Gordon JI
通讯作者：
Gordon JI
DOI：
10.1073/pnas.130568297
发表时间：
2000-06-20
影响因子：
11.1
作者：
Sorensen, SO;Pauly, M;Ulvskov, P
通讯作者：
Ulvskov, P