Profiling of adrenocorticotropic hormone and arginine vasopressin in human pituitary gland and tumor thin tissue sections using droplet-based liquid-microjunction surface-sampling-HPLC-ESI-MS-MS.
Profiling of adrenocorticotropic hormone and arginine vasopressin in human pituitary gland and tumor thin tissue sections using droplet-based liquid-microjunction surface-sampling-HPLC-ESI-MS-MS.
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使用基于液滴的液体微结S采样 - ESI-MS-MS-MS-MS-MS-MS在人垂体和肿瘤薄组织切片中，肾上腺皮质激素和精氨酸加压素的分析。
DOI：
10.1007/s00216-015-8803-2
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发表时间：
2015-08
影响因子：
4.3
通讯作者：
Van Berkel GJ
中科院分区：
文献类型：
--
作者：
Kertesz V;Calligaris D;Feldman DR;Changelian A;Laws ER;Santagata S;Agar NY;Van Berkel GJ
本文描述了使用全自动基于液滴的液体微结表面采样-HPLC-ESI-MS/MS系统对正常人垂体和垂体瘤组织切片中的蛋白质精氨酸加压素（AVP）和促肾上腺皮质激素（ACTH）进行分析的结果。空间分辨采样、HPLC分离和质谱检测。良好的相关性之间的蛋白质分布的数据与此液滴为基础的液体微交界面采样-HPLC-ESI-MS/MS系统和基质辅助激光解吸电离（MALDI）化学成像分析的同一组织的连续切片获得的数据。蛋白质分布与垂体的可见解剖结构相关。AVP在垂体后叶区（神经垂体）最丰富，ATCH在垂体前叶区（腺垂体）占优势。从一系列ACTH分泌和非分泌垂体腺瘤中取样的AVP和ACTH的相对量与组织病理学评价相关。与非分泌性腺瘤和神经垂体相比，在ACTH分泌性腺瘤和正常腺垂体前叶中很容易检测到ACTH水平显著较高。AVP多见于正常神经垂体。这项工作表明，一个完全自动化的液滴为基础的液体微连接表面采样系统耦合到HPLC-ESI-MS/MS可以很容易地用于空间分辨采样，分离，检测和半定量生理相关的肽和蛋白质激素，如AVP和ACTH，直接从人体组织。此外，当前方法的相对简单性、快速性和特异性支持了这种基础技术的潜力，进一步促进了辅助手术决策。
Described here are the results from the profiling of the proteins arginine vasopressin (AVP) and adrenocorticotropic hormone (ACTH) from normal human pituitary gland and pituitary adenoma tissue sections using a fully automated droplet-based liquid microjunction surface sampling-HPLC-ESI-MS/MS system for spatially resolved sampling, HPLC separation, and mass spectral detection. Excellent correlation was found between the protein distribution data obtained with this droplet-based liquid microjunction surface sampling-HPLC-ESI-MS/MS system and those data obtained with matrix assisted laser desorption ionization (MALDI) chemical imaging analyses of serial sections of the same tissue. The protein distributions correlated with the visible anatomic pattern of the pituitary gland. AVP was most abundant in the posterior pituitary gland region (neurohypophysis) and ATCH was dominant in the anterior pituitary gland region (adenohypophysis). The relative amounts of AVP and ACTH sampled from a series of ACTH secreting and non-secreting pituitary adenomas correlated with histopathological evaluation. ACTH was readily detected at significantly higher levels in regions of ACTH secreting adenomas and in normal anterior adenohypophysis compared to non-secreting adenoma and neurohypophysis. AVP was mostly detected in normal neurohypophysis as anticipated. This work demonstrates that a fully automated droplet-based liquid microjunction surface sampling system coupled to HPLC-ESI-MS/MS can be readily used for spatially resolved sampling, separation, detection, and semi-quantitation of physiologically-relevant peptide and protein hormones, such as AVP and ACTH, directly from human tissue. In addition, the relative simplicity, rapidity and specificity of the current methodology support the potential of this basic technology with further advancement for assisting surgical decision-making.