Biodegradable spirulina extract/polycaprolactone porous scaffolds
Biodegradable spirulina extract/polycaprolactone porous scaffolds
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DOI：
10.1039/c8nj01617h
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发表时间：
2018-09
影响因子：
3.3
通讯作者：
S. Bo;Liming Zhang;L. Liang;Jianfeng Ban
中科院分区：
文献类型：
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作者：
S. Bo;Liming Zhang;L. Liang;Jianfeng Ban
亲水性、多孔互联结构和可降解性是组织工程支架的重要特性。通过颗粒浸出法将螺旋藻提取物（SP-E）引入聚己内酯（PCL）中，得到SP-E/PCL支架，并对其孔隙度、亲水性、吸水能力和可降解性进行表征。显微分析表明，由于制备过程中SP-E的浸出，孔隙尺寸在250 ~ 300 μm之间，大孔隙中存在细小的连通孔。引入SP-E后，SP-E/PCL支架的亲水性明显增强。SP-E/PCL支架的水接触角在2 s时从120°减小到65°，7 s时进一步减小到0°。吸水能力由原来重量的2.5倍提高到8.5倍。随着SP-E添加量的增加，SP-E/PCL支架降解迅速，PBS浸泡25 d后，由于SP-E和PCL的共降解，重量损失率从2.2% （PCL支架）增加到29.2% （20 wt% SP-E/PCL支架）。SP-E/PCL-20降解28 d后分子量变化不大。降解后的SP-E/PCL-20样品在28 d后形成羟基，并且随着SP-E用量的增加，PBS浸泡25 d后支架表面形成的微孔数量增加。使用SP-E作为次级相改善了孔隙度、连通性、亲水性和吸水能力，并加速了SP-E与PCL之间的降解。这些结果表明SP-E/PCL支架在组织工程中具有潜在的应用前景。
Hydrophilicity, pores with interconnected structures, and degradability are important properties of tissue engineering scaffolds. The degradability after introducing spirulina extract (SP-E) to polycaprolactone (PCL) via particulate leaching to obtain SP-E/PCL scaffolds was characterized on the basis of their porosity, hydrophilicity, water absorption capacity, and degradability. Microscopy analysis showed that the pore sizes were in the range of 250–300 μm including small interconnected pores within large pores because of some SP-E leaching out during the fabrication process. The hydrophilicity of the SP-E/PCL scaffold was considerably enhanced by introducing SP-E. The water contact angles of the SP-E/PCL scaffold decreased from 120° to 65° at 2 s followed by further decrease to 0° at 7 s. The water absorption capacity increased from 2.5 to 8.5 times the original weight. SP-E/PCL scaffolds quickly degraded with increasing amounts of SP-E, and the percentage of weight loss increased from 2.2% (PCL scaffold) to 29.2% (20 wt% SP-E/PCL scaffold) after immersion in PBS for 25 d because of the co-degradation of SP-E and PCL. The molecular weight of SP-E/PCL-20 changed slightly after only 28 d degradation. The hydroxyl groups of the degraded SP-E/PCL-20 specimen were formed after 28 d. Moreover, the number of micropores formed on the surface of the scaffold after immersion in PBS for 25 d increased with increasing amounts of SP-E. Using SP-E as a secondary phase improved porosity, interconnectivity, hydrophilicity, and water absorption capacity and caused accelerated degradation between SP-E and PCL. These findings suggest that SP-E/PCL scaffolds have potential applications in tissue engineering.