Use of the peroxidase mimetic activity of erythrocyte-like Cu1.8S nanoparticles in the colorimetric determination of glutathione
Use of the peroxidase mimetic activity of erythrocyte-like Cu1.8S nanoparticles in the colorimetric determination of glutathione
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DOI：
10.1039/c6ay03034c
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发表时间：
2017-02
影响因子：
3.1
通讯作者：
H. Zou;Tong Yang;J. Lan;C. Huang
中科院分区：
文献类型：
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作者：
H. Zou;Tong Yang;J. Lan;C. Huang
采用溶剂热法一步合成了红细胞样硫化铜纳米颗粒（Cu1.8S NPs）。所制备的Cu1.8S纳米颗粒在近红外区域表现出局部的表面等离子体共振，由于其铜的缺乏，这导致了优异的过氧化物酶模拟性能。在H2 O2存在下，经过芬顿反应后，Cu1.8S纳米颗粒能够催化四甲基联苯胺生成蓝色氧化产物。谷氨酸通过清除Cu 1. 8 S-H2 O2体系中产生的活性氧来抑制Cu 1. 8 S纳米粒的类过氧化物酶活性。因此，基于红细胞样Cu1.8S纳米颗粒的过氧化物酶模拟活性，建立了谷胱甘肽的比色传感平台，线性范围为0.5-10 mM，检测限为0.06 mM。该阵列已成功地用于商业药物片剂中谷胱甘肽的测定。
Erythrocyte-like copper sulfide nanoparticles (Cu1.8S NPs) were successfully synthesized through a facile one-step solvothermal process. The as-prepared Cu1.8S NPs exhibited localized surface plasmon resonance in the near-infrared region due to their copper deficiency, which resulted in excellent peroxidase mimetic properties. The Cu1.8S NPs were able to catalyse tetramethyl benzidine to its blue oxidized product in the presence of H2O2 following the Fenton reaction. Glutathione inhibited the peroxidase-like activity of Cu1.8S NPs by scavenging the reactive oxygen species produced in the Cu1.8S–H2O2 system. Thus a colorimetric sensing platform for glutathione was established with a linear range of 0.5–10 mM and a limit of detection of 0.06 mM based on the peroxidase mimetic activity of erythrocyte-like Cu1.8S NPs. The array was successfully used for the determination of glutathione in commercial pharmaceutical tablets.