ENTRY OF LISTERIA-MONOCYTOGENES INTO HEPATOCYTES REQUIRES EXPRESSION OF INLB, A SURFACE PROTEIN OF THE INTERNALIN MULTIGENE FAMILY
ENTRY OF LISTERIA-MONOCYTOGENES INTO HEPATOCYTES REQUIRES EXPRESSION OF INLB, A SURFACE PROTEIN OF THE INTERNALIN MULTIGENE FAMILY
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DOI：
10.1111/j.1365-2958.1995.tb02297.x
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发表时间：
1995-04-01
影响因子：
3.6
通讯作者：
COSSART, P
中科院分区：
文献类型：
--
作者：
DRAMSI, S;BISWAS, I;COSSART, P
单核细胞增多性李斯特菌（Listeria monocytogenes）是一种细胞内细菌，它能侵入几种正常非吞噬细胞，进入培养的上皮细胞需要inlA基因的表达，inlA是一个操纵子的第一个基因，它包含两个基因：inlA和inlB，inlA编码一种由800个氨基酸组成的蛋白质，inlB编码一种由630个氨基酸组成的蛋白质。InlB是其中之一。通过构建inlAB基因座的染色体缺失突变体，我们的研究结果表明：i）inlB是进入单核细胞增多症所必需的;这些结果首次揭示了单核细胞增生李斯特菌的细胞嗜性。单核细胞增多症
The intracellular bacterium Listeria monocytogenes can invade several types of normally non-phagocytic cells, Entry into cultured epithelial cells requires the expression of inlA, the first gene of an operon, comprising two genes: inlA, which encodes internalin, an 800-amino-acid protein, and inlB, which encodes a 630-amino-acid protein, Several genes homologous to inlA are detected in the genome of L. monocytogenes; InlB is one of them, We have assessed the role of inlB in invasiveness of L. monocytogenes by constructing isogenic chromosomal deletion mutants in the inlAB locus, Our findings indicate that: i) inlB is required for entry of L. monocytogenes into hepatocytes, but not into intestinal epithelial cells; ii) inlB encodes a surface protein; iii) internalin plays a role for entry into some hepatocyte cell lines, These results provide the first insight into the cell tropism displayed by L. monocytogenes.