In-vitro circadian rhythm of murine bone marrow progenitor production
In-vitro circadian rhythm of murine bone marrow progenitor production
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DOI：
10.1081/cbi-120002677
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发表时间：
2002-01-01
影响因子：
2.8
通讯作者：
Lévi, F
中科院分区：
文献类型：
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作者：
Bourin, P;Ledain, AF;Lévi, F
啮齿动物和人类的造血过程都表现出 24 小时节律。我们假设这些节律部分是由骨髓内的昼夜节律振荡器产生的。在长达 198 小时的时间内，每 3 小时采集一次液体培养样品，研究小鼠骨髓粒单核细胞 (GM) 前体在集落刺激因子 (rm GM-CSF) 暴露后形成集落的能力。 CFU-GM 计数在培养的前 4 天内有节奏地变化，在清晨时达到可重复的最大值，类似于体内观察到的情况。这些实验提供了骨髓祖细胞在体外维持昼夜节律的第一个证据，并且证明了这些细胞内存在昼夜节律计时系统。结果支持骨髓培养物在研究抗癌药物和细胞因子对该靶系统的时间药理学作用方面的潜在用途。版权所有 (C) 2002，Marcel Dekker, Inc.
Hematopoietic processes display 24h rhythms both in rodents and in human beings. We hypothesized these rhythms to be in part generated by a circadian oscillator within the bone marrow. The ability of murine bone marrow granulo-monocytic (GM) precursors to form colonies following colony-stimulating factor (rm GM-CSF) exposure was investigated in liquid culture samples obtained every 3 h for a span of up to 198 h. The CFU-GM count varied rhythmically over the first 4 d of culture, with a reproducible maximum in the early morning hours, similar to that observed in vivo. These experiments provide the first evidence that bone marrow progenitors sustain in vitro circadian rhythmicity, and they demonstrate the presence of a circadian timekeeping system within these cells. The results support the potential usefulness of bone marrow cultures for investigating chronopharmacologic effects of anticancer drugs and cytokines on this target system. Copyright (C) 2002 by Marcel Dekker, Inc.