Subspecialization of CXCR5+ T cells: B helper activity is focused in a germinal center-localized subset of CXCR5+ T cells.
Subspecialization of CXCR5+ T cells: B helper activity is focused in a germinal center-localized subset of CXCR5+ T cells.
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DOI：
10.1084/jem.193.12.1373
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发表时间：
2001-06-18
影响因子：
15.3
通讯作者：
Butcher, E C
中科院分区：
文献类型：
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作者：
Kim, C H;Rott, L S;Clark-Lewis, I;Campbell, D J;Wu, L;Butcher, E C
辅助性T（Th）细胞库由具有异质效应子功能的特化细胞组成。除了Th 1和Th 2细胞之外，CXCR 5 + T细胞被认为是另一种专门用于B细胞帮助的效应T细胞。我们在这里表明，CXCR 5 + T细胞是异质性的，我们确定了CXCR 5 + CD 4 T细胞的亚群，不同的功能和微环境定位在次级淋巴组织。CD 57 + CXCR 5 T细胞，以下称为生发中心Th（GC-Th）细胞，仅位于GC中，缺乏CCR 7，并且对滤泡趋化因子B淋巴细胞化学引诱物高度应答，但对T细胞区EBI 1配体趋化因子不应答。重要的是，GC-Th细胞在诱导B细胞产生抗体方面比CD 57 − CXCR 5 + T细胞或CXCR 5 − T细胞更有效。与它们的功能一致，GC-Th细胞在刺激后产生升高水平的白细胞介素10，与其他细胞因子和共刺激分子一起，可能有助于赋予它们的B细胞辅助活性。我们的研究结果表明，CXCR 5 + T细胞是功能异质性的，GC-Th细胞，CXCR 5 + T细胞的一个小的子集，是淋巴组织中B细胞分化和抗体产生的关键助手。
The T helper (Th) cell pool is composed of specialized cells with heterogeneous effector functions. Apart from Th1 and 2 cells, CXCR5+ T cells have been suggested to be another type of effector T cell specialized for B cell help. We show here that CXCR5+ T cells are heterogeneous, and we identify subsets of CXCR5+ CD4 T cells that differ in function and microenvironmental localization in secondary lymphoid tissues. CD57+CXCR5 T cells, hereafter termed germinal center Th (GC-Th) cells, are localized only in GCs, lack CCR7, and are highly responsive to the follicular chemokine B lymphocyte chemoattractant but not to the T cell zone EBI1-ligand chemokine. Importantly, GC-Th cells are much more efficient than CD57−CXCR5+ T cells or CXCR5− T cells in inducing antibody production from B cells. Consistent with their function, GC-Th cells produce elevated levels of interleukin 10 upon stimulation which, with other cytokines and costimulatory molecules, may help confer their B cell helper activity. Our results demonstrate that CXCR5+ T cells are functionally heterogeneous and that the GC-Th cells, a small subset of CXCR5+ T cells, are the key helpers for B cell differentiation and antibody production in lymphoid tissues.