Analysis of polypeptide expression in benign and malignant human breast lesions
Analysis of polypeptide expression in benign and malignant human breast lesions
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DOI：
10.1002/elps.1150180341
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发表时间：
1997-03-01
期刊：
影响因子：
2.9
通讯作者：
Auer, G
中科院分区：
文献类型：
--
作者：
Franzen, B;Linder, S;Auer, G
本文报道了人乳腺癌的双向电泳(2-DE)结果。从不同增殖指数和基因组稳定程度的乳腺癌中提取和纯化肿瘤细胞。从纤维腺瘤组织中提纯的细胞作为良性细胞的对照。研究结果如下：(1)同一肿瘤不同部位的多肽表达模式具有高度相似性，两种肿瘤及其转移灶的双向凝胶电泳图谱相似。(Ii)相反，具有相似组织学特征的不同病变之间的多肽表达差异很大，与恶性程度较低的病变相比，潜在的高度恶性癌之间的多肽表达差异更大。这些差异与将乳腺癌与肺癌进行比较所观察到的差异在同一量级。(Iii)所有细胞角蛋白形式(CK7、CK8、CK15和CK18)在癌中的分解水平显著低于纤维腺瘤。(4)大分子原肌球蛋白(1-3)在癌组织中的表达低于纤维腺瘤，原肌球蛋白-L在有腋窝淋巴结转移的原发肿瘤中的表达是无转移的原发肿瘤的1.7倍(P<；0.05)；(5)增殖细胞核抗原及其应激蛋白家族的一些成员(PHSP60、HSP90和钙网蛋白)在癌组织中的表达较高。我们的结论是，乳腺癌的恶性进展导致不同肿瘤之间多肽表达的高度异质性，但也可以辨别出一些共同的主题，如细胞角蛋白和原肌球蛋白多肽的表达减少。
Results of two-dimensional electrophoresis (2-DE) analyses of human breast carcinoma are described. Tumor cells were extracted and purified from breast carcinomas with different proliferative indeces and degrees of genomic stability. Cells purified from fibroadenoma tissue served as controls for benign cells. The following results were observed: (i) Analysis of samples from different areas of the same tumor showed a high degree of similarity in the pattern of polypeptide expression, Similarly, analysis of two tumors and their metastases revealed similar 2-DE profiles. (ii) In contrast, large variations were observed between different lesions with comparable histological characteristics, Larger differences in polypeptide expression were observed between potentially highly malignant carcinomas compared to comparisons of less malignant lesions. These differences were in the same order of magnitude as those observed comparing a breast carcinoma to a lung carcinoma. (iii) The levels of all cytokeratin forms resolved (CK7, CK8, CK15, and CK18) were significantly lower in carcinomas compared to fibroadenomas. (iv) The levels of high molecular weight tropomyosins (1-3) were lower in carcinomas compared to fibroadenomas, The expression of tropomyosin-l was found to be 1.7-fold higher in primary tumors with metastatic spread to axillar lymph nodes compared to primary tumors with no evidence of metastasis (p < 0.05), (v) The expression of proliferating cell nuclear antigen (PCNA) and some members of the stress protein family (pHSP60, HSP90, and calreticulin) were higher in carcinomas. We conclude that malignant progression of breast carcinomas results in large heterogeneity in polypeptide expression between different tumors, but that some common themes such as decreased expression of cytokeratin and tropomyosin polypeptides can be discerned.