New restriction endonuclease CviRI cleaves DNA at TG/CA sequences.
New restriction endonuclease CviRI cleaves DNA at TG/CA sequences.
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新的限制性内切酶 CviRI 可在 TG/CA 序列处切割 DNA。
DOI：
10.1093/nar/22.19.3928
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发表时间：
1994
影响因子：
14.9
通讯作者：
VanEtten,JL
中科院分区：
文献类型：
--
作者：
Jin,A;Zhang,Y;Xia,Y;Traylor,E;Nelson,M;VanEtten,JL
从病毒XZ-6E感染的小球藻细胞中分离出一种新型II型限制性内切酶CviRI。CviRI是第一个识别序列5'-TGCA-3'的限制性内切酶，并在G和C残基之间切割DNA以产生平末端。 5'-TGCA-3' 序列中腺嘌呤或胞嘧啶的甲基化可防止 CviRI 裂解。由于其序列特异性，CviRI 对于检测许多人类遗传性疾病的突变等位基因特别有用。
A new type II restriction endonuclease,CviRI, was isolated from virus XZ-6E infected chlorella cells.CviRI is the first restriction endonuclease to recognize the sequence 5′-TGCA-3′ and cleaves DNA between the G and C residues to produce blunt-end termini. Methylation of the adenine or cytosine in 5′-TGCA-3′ sequences preventsCviRI cleavage. Due to its sequence specificity,CviRI may be especially useful for detecting mutant alleles of many heritable human genetic diseases.