Imaging mass spectrometry distinguished the cancer and stromal regions of oral squamous cell carcinoma by visualizing phosphatidylcholine (16:0/16:1) and phosphatidylcholine (18:1/20:4)
Imaging mass spectrometry distinguished the cancer and stromal regions of oral squamous cell carcinoma by visualizing phosphatidylcholine (16:0/16:1) and phosphatidylcholine (18:1/20:4)
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DOI：
10.1007/s00216-013-7062-3
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发表时间：
2014-02-01
影响因子：
4.3
通讯作者：
Setou, Mitsutoshi
中科院分区：
文献类型：
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作者：
Uchiyama, Yoshiyuki;Hayasaka, Takahiro;Setou, Mitsutoshi
大多数口腔癌是口腔鳞状细胞癌（OSCC）。用苏木精和伊红对OSCC的解剖特征进行了组织化学评价。然而，癌症和间质区之间的界限不清楚，大部分癌症和间质区在手术中被切除。为了减少切除面积和维持口腔功能，需要一种新的诊断方法。在这项研究中，我们试图在成像质谱（IMS）可视化的生物分子分布的基础上明确区分边界。在IMS数据集中，11个信号在癌区和间质区之间的强度有显著差异（p < 0.01）。各区域分布m/z 770.5和m/z 846.6两个信号，边界清晰。串联质谱（MS/MS）分析鉴定出这些信号分别为：肿瘤区m/z 770.5处的磷脂酰胆碱（PC）（16:0/16:1）和基质区m/z 846.6处的磷脂酰胆碱（PC）（18:1/20:4）。此外，含有花生四烯酸的PC种在基质区域的分布表明，淋巴细胞的积累是对癌症侵袭引起的炎症的反应。综上所述，利用这些PC种和IMS分析可以清晰地区分OSCCs的癌区和间质区，这一分子鉴定可以为阐明肿瘤侵袭机制提供重要信息。
Most oral cancers are oral squamous cell carcinoma (OSCC). The anatomical features of OSCC have been histochemically evaluated with hematoxylin and eosin. However, the border between the cancer and stromal regions is unclear and large portions of the cancer and stromal regions are resected in surgery. To reduce the resected area and maintain oral function, a new method of diagnosis is needed. In this study, we tried to clearly distinguish the border on the basis of biomolecule distributions visualized by imaging mass spectrometry (IMS). In the IMS dataset, eleven signals were significantly different in intensity (p < 0.01) between the cancer and stromal regions. Two signals at m/z 770.5 and m/z 846.6 were distributed in each region, and a clear border was revealed. Tandem mass spectrometric (MS/MS) analysis identified these signals as phosphatidylcholine (PC) (16:0/16:1) at m/z 770.5 in the cancer region and PC (18:1/20:4) at m/z 846.6 in the stromal region. Moreover, the distribution of PC species containing arachidonic acid in the stromal region suggests that lymphocytes accumulated in response to the inflammation caused by cancer invasion. In conclusion, the cancer and stromal regions of OSCCs were clearly distinguished by use of these PC species and IMS analysis, and this molecular identification can provide important information to elucidate the mechanism of cancer invasion.