Splicing Variation at a FLOWERING LOCUS C Homeolog Is Associated With Flowering Time Variation in the Tetraploid Capsella bursa-pastoris
Splicing Variation at a FLOWERING LOCUS C Homeolog Is Associated With Flowering Time Variation in the Tetraploid Capsella bursa-pastoris
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开花位点 C 同源物的剪接变异与四倍体荠菜的开花时间变异相关
DOI：
10.1534/genetics.109.103705
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发表时间：
2009-09-01
期刊：
影响因子：
3.3
通讯作者：
Lascoux, Martin
中科院分区：
文献类型：
--
作者：
Slotte, Tanja;Huang, Hui-Run;Lascoux, Martin
重复基因的长期命运在经验上和理论上都得到了很好的研究，但是重复基因的短期进化如何促成表型变异却鲜为人知。本文研究了二体四倍体荠菜开花时间变异的遗传基础。我们对来自欧亚大陆西部和中国的样本中的四个重复的开花时间候选基因和10个背景基因座进行了测序。使用混合模型的方法，占人口结构，我们发现，多态性在一个同源的两个候选基因，基因座C（FLC）和基因座1（FLC 1），与自然开花时间的变化。没有潜在的致病多态性被发现在编码区的FLC 1，但是，在两个剪接位点多态性与早花。包含非共有剪接位点的接入表达了选择性剪接转录或不表达此FLC同源物。我们的研究结果是一致的功能FLC作为一个主要的抑制开花拟南芥，并意味着重复基因的非功能化可以提供一个重要的来源的表型变异。
The long-term fates of duplicate genes are well studied both empirically and theoretically, but how the short-term evolution of duplicate genes contributes to phenotypic variation is less well known. Here, we have studied the genetic basis of flowering time variation in the disomic tetraploid Capsella bursa-pastoris. We sequenced four duplicate candidate genes for flowering time and 10 background loci in samples from western Eurasia and China. Using a mixed-model approach that accounts for population structure, we found that polymorphisms at one homeolog of two candidate genes, FLOWERING LOCUS C (FLC) and CRYPTOCHROME1 (CRY1), were associated with natural flowering time variation. No potentially causative polymorphisms were found in the coding region of CRY1; however, at FLC two splice site polymorphisms were associated with early flowering. Accessions harboring nonconsensus splice sites expressed an alternatively spliced transcript or did not express this FLC homeolog. Our results are consistent with the function of FLC as a major repressor of flowering in Arabidopsis thaliana and imply that nonfunctionalization of duplicate genes could provide an important source of phenotypic variation.