Cardiorespiratory fitness modulates the proportions of monocytes and T helper subsets in lean and obese men
Cardiorespiratory fitness modulates the proportions of monocytes and T helper subsets in lean and obese men
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DOI：
10.1111/sms.13506
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发表时间：
2019-07-11
影响因子：
4.1
通讯作者：
Romao, Pedro R. T.
中科院分区：
文献类型：
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作者：
Dorneles, Gilson P.;da Silva, Igor;Romao, Pedro R. T.
本研究探讨了不同心肺功能（CRF）水平的瘦和肥胖男性外周血单核细胞频率、CD 4 + T细胞亚群和全身细胞因子水平。从45名瘦男性和45名肥胖男性中获得单核细胞，根据他们的体重指数和CRF（低、中或高VO 2 Peak）将他们分为六组，以分析单核细胞亚群和CD 4 + T细胞亚群（Treg细胞，CD 4 + CD 25（高）CD 127（低）; mTeff，CD 4 + CD 25-CD 39 +; mTreg，CD 4 + CD 25 + CD 39+）的频率。还评价了白细胞介素（IL）-6，IL-10，IL-17 a，IL-33，瘦素和肿瘤坏死因子-α（TNF-α）的全身水平。7名久坐不动的肥胖男性进行了一周的高强度间歇训练（HIIT，3次/周），并在最后一次训练之前和之后24小时收集血液样本用于T细胞和单核细胞的表型分析。肥胖个体呈现出以较低频率的Treg和mTreg细胞和较高比例的促炎性单核细胞为特征的炎症特征。然而，较高的CRF状态增加Treg细胞和mTreg细胞的频率，并降低CD 4 + mTeff细胞和中间和非经典单核细胞在瘦和肥胖男性外周血中的百分比。在所有受试者中，在中度和重度CRF中观察到全身促炎性（IL-6和TNF-）细胞因子水平较低，IL-10和IL-33浓度较高。HIIT增加了久坐肥胖个体中循环mTreg和Treg细胞的比例。CRF的免疫调节作用有助于维持低水平的炎症介质。
This study investigated the peripheral frequency of monocytes, CD4 + T cell subsets and the systemic levels of cytokines in lean and obese men with different levels of cardiorespiratory fitness (CRF). Mononuclear cells were obtained from 45 lean and 45 obese men who were assigned into six groups according to their body mass index and CRF (low, moderate, or high VO2Peak) to analyze the frequency of monocyte subsets and subpopulations of CD4 + T cells (Treg cells, CD4 + CD25(high)CD127(low); mTeff, CD4 + CD25-CD39+; mTreg, CD4 + CD25+CD39+). The systemic levels of interleukin (IL)-6, IL-10, IL-17a, IL-33, leptin, and tumor necrosis factor-alpha (TNF-alpha) were also evaluated. Seven sedentary obese men performed one week of high-intensity interval training (HIIT, 3 sessions/week), and blood samples were collected before and 24 hours after the last session for phenotypic analysis of T cells and monocytes. Obese individuals presented an inflammatory profile characterized by lower frequencies of Treg and mTreg cells and higher proportions of proinflammatory monocytes. However, higher CRF status increased the frequencies of Treg cells and mTreg cells and decreased the percentage of CD4 + mTeff cells and intermediate and non-classical monocytes in the peripheral blood from lean and obese men. Systemic lower levels of proinflammatory (IL-6 and TNF-) cytokines and higher concentrations of IL-10 and IL-33 were observed in moderate and higher CRF in all subjects. HIIT increased the proportions of circulating mTreg and Treg cells in sedentary obese individuals. The immunoregulatory role of CRF contributes to the maintenance of low levels of inflammatory mediators.