Exogenous malic acid alleviates cadmium toxicity in Miscanthus sacchariflorus through enhancing photosynthetic capacity and restraining ROS accumulation
Exogenous malic acid alleviates cadmium toxicity in Miscanthus sacchariflorus through enhancing photosynthetic capacity and restraining ROS accumulation
复制标题
外源苹果酸通过增强光合能力和抑制ROS积累减轻芒草镉毒性
DOI：
10.1016/j.ecoenv.2017.03.018
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发表时间：
2017-07-01
影响因子：
6.8
通讯作者：
Zheng, Bingsong
中科院分区：
文献类型：
--
作者：
Guo, Haipeng;Chen, Houming;Zheng, Bingsong
苹果酸（MA）在植物生长、气孔开度、营养元素平衡和有毒金属耐性等方面具有重要的调节作用。然而，外源MA对植物对有毒金属的生理生化反应的影响却知之甚少。为探讨外源MA对镉（Cd）胁迫的缓解作用，研究了MA对Miscatzthus sacchariflorus（M.蔗糖花属（sacchariflorus））植物对Cd胁迫的响应。单独Cd处理显著抑制了植株的生长和Pn，但增加了ROS的积累，尽管抗氧化酶被显著激活。糖花。与单独Cd胁迫相比，MA处理显著促进了植株生长，降低了Cd积累量，同时提高了Pn，尤其是高浓度MA（200 μ M）处理。此外，Cd和MA具有协同效应，可进一步提高部分抗氧化酶的活性和基因表达，从而增加谷胱甘肽的积累，减少ROS的产生。结果表明，施用MA可通过调节Cd胁迫下的酶和非酶抗氧化剂来减轻Cd诱导的植物毒性和氧化损伤。糖花。
Malic acid (MA) plays an important role in the regulation of plant growth, stomatal aperture, nutrition elements homeostasis and toxic metals tolerance. However, little is known about the effects of exogenous MA on physiological and biochemical responses to toxic metals in plants. To measure the alleviation roles of exogenous MA against cadmium (Cd), we determined the effects of MA on plant growth, net photosynthetic rate (Pn), reactive oxygen species (ROS) accumulation and the activities of anti-oxidant enzymes in the leaves of Miscatzthus sacchariflorus (M. sacchariflorus) under Cd stress. The Cd exposure alone significantly inhibited plant growth and Pn, but increased the accumulation of ROS even though the anti-oxidant enzymes were markedly activated in the leaves of M. sacchariflorus. Treatment with MA significantly enhanced plant growth and decreased Cd accumulation accompanied by increasing Pn under Cd stress as compared to Cd stress alone, especially when treatment with high concentration of MA (200 mu M) was used. In addition, Cd and MA indicated synergistic effects by further increasing the activities and genes expression of partial anti-oxidant enzymes, thus resulting in higher glutathione accumulation and reduction of ROS production. The results showed that application of MA alleviated Cd-induced phytotoxicity and oxidant damage through the regulation of both enzymatic and non-enzymatic anti-oxidants under Cd stress in M. sacchariflorus.