Protection against aerosolized Yersinia pestis challenge following homologous and heterologous prime-boost with recombinant plague antigens
Protection against aerosolized Yersinia pestis challenge following homologous and heterologous prime-boost with recombinant plague antigens
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DOI：
10.1128/iai.73.8.5256-5261.2005
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发表时间：
2005-08-01
影响因子：
3.1
通讯作者：
Clements, JD
中科院分区：
文献类型：
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作者：
Glynn, A;Roy, CJ;Clements, JD
一种鼠疫耶尔森菌衍生的融合蛋白（FIN）在小鼠研究中显示出作为一种抗鼠疫耶尔森菌气溶胶攻击的保护性抗原的巨大希望。在目前的研究中，我们研究了不同的F1-V启动-增强方案，并证明(i)与启动剂量不同的途径（异种增强）可以保护小鼠免受鼠疫菌气溶胶攻击，（ii）不需要肠外免疫来保护小鼠免受雾化鼠疫攻击。（iii）免疫途径和佐剂的选择影响抗体反应的大小以及免疫球蛋白G1 (IgG1)/IgG2a的比率，（iv）适当的佐剂对非肠外免疫至关重要。
A Yersinia pestis-derived fusion protein (FIN) has shown great promise as a protective antigen against aerosol challenge with Y. pestis in murine studies. In the current study, we examined different prime-boost regimens with F1-V and demonstrate that (i) boosting by a route other than the route used for the priming dose (heterologous boosting) protects mice as well as homologous boosting against aerosol challenge with Y pestis, (ii) parenteral immunization is not required to protect mice against aerosolized plague challenge, (iii) the route of immunization and choice of adjuvant influence the magnitude of the antibody response as well as the immunoglobulin G1 (IgG1)/IgG2a ratio, and (iv) inclusion of an appropriate adjuvant is critical for nonparenteral immunization.