Altitude-scale variation in nitrogen-removal bacterial communities from municipal wastewater treatment plants distributed along a 3600-m altitudinal gradient in China
Altitude-scale variation in nitrogen-removal bacterial communities from municipal wastewater treatment plants distributed along a 3600-m altitudinal gradient in China
复制标题
中国3600米海拔梯度城镇污水处理厂脱氮细菌群落海拔尺度变化
DOI：
10.1016/j.scitotenv.2016.03.175
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发表时间：
--
影响因子：
9.8
通讯作者：
Linqiong Wang
中科院分区：
文献类型：
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作者：
Lihua Niu;Yi Li;Peifang Wang;Wenlong Zhang;Chao Wang;Wei Cai;Linqiong Wang
污水处理厂脱氮过程的微生物生态信息作为诊断脱氮效果差的手段具有相当重要的意义。本文研究了分布在海拔3660米的中国18个城市污水处理厂中含铜亚硝酸盐还原酶基因（nirK-NRB）和含细胞色素1亚硝酸盐还原酶基因（nrs - nrb）的β变形杆菌氨氧化菌（βAOB）和亚硝酸盐还原菌的数量关系和群落结构的海拔变化。在海拔约1500 m处检测到NRB与总细菌、NRB与βAOB、nirk -NRB与s -NRB的比例相关的海拔阈值。与1500海拔高度以下的稳定比例相比，1500海拔高度以上的比例随海拔高度的增加呈明显的下降趋势。Spearman相关分析表明，这一趋势受海拔高度以及多种废水和操作变量的显著驱动。βAOB、nirK-NRB和nirs - nrb群落结构差异与湿地间海拔高度呈显著正相关。冗余分析表明，1500 masl以上群落结构的变化主要与废水有关，其次是操作和海拔。综上所述，虽然污水处理厂中除氮细菌群落的变化主要受污水和操作变量的驱动，但海拔也是影响污水处理厂中除氮细菌数量关系和群落结构的重要变量，特别是在1500立方米以上。
Microbial ecological information on the nitrogen removal processes in wastewater treatment plants (WWTPs) has been of considerable importance as a means for diagnosing the poor performance of nitrogen removal. In this study, the altitude-scale variations in the quantitative relationships and community structures of betaproteobacteria ammonia-oxidizing bacteria (βAOB) and nitrite-reducing bacteria containing the copper-containing nitrite reductase gene (nirK-NRB) and the cytochromecd1-containing nitrite reductase gene (nirS-NRB) were investigated in 18 municipal WWTPs distributed along a 3660-masl altitude gradient in China. An altitude threshold associated with the proportions of NRB to total bacteria, NRB to βAOB andnirK-NRB tonirS-NRB was detected at approximately 1500 m above sea level (masl). Compared with the stable proportions below 1500 masl, the proportions exhibited a pronounced decreasing trend with increased altitude above 1500 masl. Spearman correlation analysis indicated that the trend was significantly driven by altitude as well as multiple wastewater and operational variables. The community structure dissimilarity of βAOB,nirK-NRB andnirS-NRB showed significant and positive correlations with altitudinal distance between WWTPs. Redundancy analyses indicated that the variation in community structures above 1500 masl were predominantly associated with wastewater, followed by operation and altitude. In summary, although the variations of nitrogen-removal bacterial community in WWTPs were driven dominantly by wastewater and operational variables, altitude was also an important variable influencing the quantitative relationships and community structures of nitrogen-removal bacteria in WWTPs particularly above 1500 masl.