Reduced expression of kinase-associated phosphatase in cortical dendrites of MAP2-deficient mice
Reduced expression of kinase-associated phosphatase in cortical dendrites of MAP2-deficient mice
复制标题
DOI：
10.1016/j.bbrc.2005.10.077
复制
发表时间：
2005-12-16
影响因子：
3.1
通讯作者：
Harada, A
中科院分区：
文献类型：
--
作者：
Iriuchijima, N;Sato-Harada, R;Harada, A
我们先前证明，在MAP2缺陷小鼠的树突中，cAMP依赖的蛋白激酶减少。在这项研究中，我们比较了野生型和MAP2(-/-)树突状细胞中各种蛋白磷酸酶(PPS)的表达。蛋白激酶相关磷酸酶(KAP)是两种表型之间唯一不同的PP：(1)MAP2(-/-)皮质树突中KAP的表达减少；(2)MAP2(-/-)脑中与微管结合的KAP数量减少。我们还在培养的神经母细胞瘤细胞中证明了KAP不仅在分裂细胞中表达，而且在分化细胞的突起中也表达。我们的发现表明，已被报道在细胞周期控制中发挥作用的KAP，在调节树突状细胞功能方面具有尚未被发现的作用。我们还建议将MAP2缺陷小鼠作为鉴定树突状细胞功能所必需的蛋白磷酸酶的理想系统。(C)2005 Elsevier Inc.保留所有权利。
We previously demonstrated that cAMP-dependent protein kinase was reduced in the dendrites of MAP2-deficient mice. In this study, we compared the expression of yarious protein phosphatases (PPs) between wild-type and map2(-/-) dendrites. Kinase-associated phosphatase (KAP) was the only PP which showed difference between the two phenotypes: (1) the expression of KAP was reduced in map2(-/-)cortical dendrites, and (2) the amount of KAP bound to microtubules was reduced in map2(-/-) brains. We also demonstrated in cultured neuroblastoma cells that KAP is not only expressed in dividing cells, but also in the neurites of differentiated cells. Our findings propose that KAP, which has been reported to function in cell-cycle control, has an as yet uncovered role in regulating dendritic functions. We also propose MAP2-deficient mice as an ideal system for identifying protein phosphatases essential for dendritic functions. (c) 2005 Elsevier Inc. All rights reserved.