Illumination of Nanoliter-NMR Spectroscopy Chips for Real-Time Photochemical Reaction Monitoring.
Illumination of Nanoliter-NMR Spectroscopy Chips for Real-Time Photochemical Reaction Monitoring.
复制标题
用于实时光化学反应监测的纳升核磁共振光谱芯片的照明。
DOI：
10.1021/acs.analchem.7b04114
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发表时间：
2018
影响因子：
7.4
通讯作者：
A. Velders
中科院分区：
文献类型：
--
作者：
M. V. Gomez;A. Juan;F. Jiménez;A. de la Hoz;A. Velders
我们报告使用一个小体积的核磁共振（NMR）光谱设备与集成光纤的紫外可见光辅助化学反应的实时检测。光纤用于将来自LED或激光二极管的光引导到25 nL检测体积，该LED或激光二极管安全地定位在磁体外部，并放置在微流体通道的正上方，照射NMR芯片的透明背面。这里提出的设置克服了传统的NMR系统的局限性，原位紫外-可见光照明，与微通道允许有效的光穿透，即使在高浓度的溶液中，需要较低的功率光强度，并实现高光子通量。的设置的功效示出了两个模型反应在不同的波长激活。
We report the use of a small-volume nuclear-magnetic-resonance (NMR)-spectroscopy device with integrated fiber-optics for the real-time detection of UV-vis-light-assisted chemical reactions. An optical fiber is used to guide the light from LEDs or a laser diode positioned safely outside the magnet toward the 25 nL detection volume and placed right above the microfluidic channel, irradiating the transparent back of the NMR chip. The setup presented here overcomes the limitations of conventional NMR systems for in situ UV-vis illumination, with the microchannel permitting efficient light penetration even in highly concentrated solutions, requiring lower-power light intensities, and enabling high photon flux. The efficacy of the setup is illustrated with two model reactions activated at different wavelengths.