A Zfp609 circular RNA regulates myoblast differentiation by sponging miR-194-5p
A Zfp609 circular RNA regulates myoblast differentiation by sponging miR-194-5p
复制标题
Zfp609 环状 RNA 通过海绵 miR-194-5p 调节成肌细胞分化
DOI：
10.1016/j.ijbiomac.2018.09.039
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发表时间：
2019-01-01
影响因子：
8.2
通讯作者：
Zhang, ChunLei
中科院分区：
文献类型：
--
作者：
Wang, YanHong;Li, MengLu;Zhang, ChunLei
骨骼肌的发育和生长调控机制是动物遗传学和医学研究的热点。近年来的研究表明，共价闭合环状RNA（circRNAs）通过隔离特定的miRNAs在肌肉发育中也发挥着重要作用。本研究旨在探讨circZfp 609在小鼠成肌细胞系（C2 G12）中的功能作用。预测circZfp 609具有miR-194- 5 p的结合位点。circZfp 609基因敲低可增加MyfS和MyoG的表达，表明circZfp 609抑制肌细胞分化。通过荧光素酶筛选测定，观察到circZfp 609海绵化至具有四个潜在结合位点的miR-194- 5 p。具体而言，我们表明circZfp 609可以海绵miR-194- 5 p，以隔离其对BCLAF 1的抑制，从而抑制肌源性分化。肌肉组织中circZfp 609表达的调节可能成为试图控制肌肉发育的育种策略的潜在目标。(C)2018由Elsevier B. V.出版
Skeletal muscle development and growth regulatory mechanism is the focus of both animal genetics and medicine. The recent studies indicate that covalently closed circular RNAs (circRNAs) also play important role on muscle development through sequestering specific miRNAs. The present study was conducted to determine the functional roles of circZfp609, a recently identified circRNA, in the regulation of myogenesis in mouse myoblast cell line (C2G12). circZfp609 is predicted to has binding sites of miR-194-5p. circZfp609 knockdown increased the expression of MyfS and MyoG, which indicated that circZfp609 suppressed myogenic differentiation. Via a luciferase screening assay, circZfp609 is observed to sponge to miR-194-5p with four potential binding sites. Specifically, we show that circZfp609 can sponge miR-194-5p to sequester its inhibition on BCLAF1 so as to repress the myogenic differentiation. Modulation of circZfp609 expression in muscle tissue may emerge as a potential target in breeding strategies attempting to control muscle development. (C) 2018 Published by Elsevier B.V.