Protective activities of the filamentous hemagglutinin and the lymphocytosis-promoting factor of Bordetella pertussis in mice.
Protective activities of the filamentous hemagglutinin and the lymphocytosis-promoting factor of Bordetella pertussis in mice.
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百日咳博德特氏菌丝状血凝素和淋巴细胞增殖促进因子对小鼠的保护活性。
DOI：
10.1093/infdis/150.6.823
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Charles R. Manciark
中科院分区：
文献类型：
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作者：
Masaaki Oda;J. Cowell;D. G. Burstyn;Charles R. Manciark
通过小鼠脑内或呼吸道攻击的主动和被动保护试验，比较了百日咳杆菌丝状血凝素（FHA）和淋巴细胞生成促进因子（LPF）的保护活性。通过腹腔注射8 μ g FHA或戊二醛灭活的LPF免疫两次的小鼠在气溶胶攻击后受到保护。一次腹腔注射灭活LPF也可保护小鼠免受脑内攻击;保护50%小鼠的剂量为8.5微克。然而，一次腹腔注射48微克FHA或每周两次腹腔注射20微克FHA并不能保护小鼠在脑内攻击后免于死亡。注射亲和纯化的抗体LPF从小鼠杂交瘤或山羊给了剂量依赖性的保护对气溶胶的挑战。最小保护剂量为80-90微克。FHA的多克隆或单克隆抗体在剂量分别为1，440 μ g或360 μ g时，在呼吸激发后对疾病的保护作用非常小。这些数据表明，小鼠主动免疫后用B进行呼吸攻击。百日咳是鉴定保护性抗原的有用模型。
Protective activities of the filamentous hemagglutinin (FHA) and the lymphocytosis-promoting factor (LPF) of Bordetella pertussis were compared by active and passive protection tests with intracerebral or respiratory challenge in mice. Mice immunized twice by intraperitoneal injection of 8 micrograms of FHA or glutaraldehyde-inactivated LPF were protected after aerosol challenge. One intraperitoneal injection of inactivated LPF also protected mice from intracerebral challenge; the dose protecting 50% of the mice was 8.5 micrograms. However, one intraperitoneal injection of 48 micrograms of FHA or two weekly intraperitoneal injections of 20 micrograms did not protect mice from death after intracerebral challenge. Injection of affinity-purified antibody to LPF from mouse hybridomas or from goats gave a dose-dependent protection against aerosol challenge. The smallest dose giving protection was 80-90 micrograms. Polyclonal or monoclonal antibody to FHA at doses of 1,440 micrograms or 360 micrograms, respectively, gave very little protection from disease after respiratory challenge. These data indicate that active immunization of mice followed by respiratory challenge with B. pertussis is a useful model to identify protective antigens.