Abundance and Community Structure of Bacteria on Asian Dust Particles Collected in Beijing, China, during the Asian Dust Season.
Abundance and Community Structure of Bacteria on Asian Dust Particles Collected in Beijing, China, during the Asian Dust Season.
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DOI：
10.1248/bpb.b15-00573
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发表时间：
2016
影响因子：
2
通讯作者：
N. Yamaguchi;T. Baba;Tomoaki Ichijo;Yuka Himezawa;Kanami Enoki;M. Saraya;Pin-Fang Li;M. Nasu
中科院分区：
文献类型：
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作者：
N. Yamaguchi;T. Baba;Tomoaki Ichijo;Yuka Himezawa;Kanami Enoki;M. Saraya;Pin-Fang Li;M. Nasu
据估计，每年约有180吨/公里（2）的亚洲沙尘颗粒落在中国北京，亚洲沙尘事件对人类健康和下风向生态系统的微生物的影响值得关注。在这项研究中，我们收集了在北京的亚洲灰尘颗粒，并分析了这些颗粒上的细菌群落的非培养的方法。首次利用激光扫描显微镜观察到亚洲沙尘颗粒上的细菌细胞，证明亚洲沙尘颗粒携带细菌细胞到北京。通过定量聚合酶链反应（PCR）测定的细菌丰度为10（8）至10（9）个细胞/g，比亚洲尘源土壤高约10倍。亚洲沙尘样品之间的细菌群落结构的季节间变异，比较终端限制性片段长度多态性（T-RFLP），在亚洲沙尘季节低。变性梯度凝胶电泳（DGGE）在亚洲灰尘样品中发现了几种活细菌，包括肠道细菌。针对16 S核糖体RNA（rRNA）基因序列的克隆文库分析表明，粉尘样品中细菌的系统发育多样性较高，且大部分为分布于土壤和空气中的环境细菌。在克隆库中的优势种是Segetibacter aerophilus（拟杆菌），这是第一次从亚洲粉尘样品中分离在韩国收集。我们的研究结果还表明，在运输过程中的细菌群落结构的变化，并增加耐干燥的细菌，如厚壁菌。
Approximately 180 t/km(2) of Asian dust particles are estimated to fall annually on Beijing, China, and there is significant concern about the influence of microbes transported by Asian dust events on human health and downwind ecosystems. In this study, we collected Asian dust particles in Beijing, and analyzed the bacterial communities on these particles by culture-independent methods. Bacterial cells on Asian dust particles were visualized first by laser scanning microscopy, which demonstrated that Asian dust particles carry bacterial cells to Beijing. Bacterial abundance, as determined by quantitative polymerase chain reaction (PCR), was 10(8) to 10(9) cells/g, a value about 10 times higher than that in Asian dust source soils. Inter-seasonal variability of bacterial community structures among Asian dust samples, as compared by terminal restriction fragment length polymorphism (T-RFLP), was low during the Asian dust season. Several viable bacteria, including intestinal bacteria, were found in Asian dust samples by denaturing gradient gel electrophoresis (DGGE). Clone library analysis targeting 16S ribosomal RNA (rRNA) gene sequences demonstrated that bacterial phylogenetic diversity was high in the dust samples, and most of these were environmental bacteria distributed in soil and air. The dominant species in the clone library was Segetibacter aerophilus (Bacteroidetes), which was first isolated from an Asian dust sample collected in Korea. Our results also indicate the possibility of a change in the bacterial community structure during transportation and increases in desiccation-tolerant bacteria such as Firmicutes.