NOVEL ACTIN CROSS-LINKER SUPERFAMILY MEMBER IDENTIFIED BY A 2-STEP DEGENERATE PCR PROCEDURE
NOVEL ACTIN CROSS-LINKER SUPERFAMILY MEMBER IDENTIFIED BY A 2-STEP DEGENERATE PCR PROCEDURE
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DOI：
10.1016/0014-5793(95)00722-l
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发表时间：
1995-07-24
期刊：
影响因子：
3.5
通讯作者：
KUNKEL, LM
中科院分区：
文献类型：
--
作者：
BYERS, TJ;BEGGS, AH;KUNKEL, LM
肌动蛋白交联蛋白将f -肌动蛋白连接到构成细胞骨架的束和网络中，肌动蛋白、肌萎缩蛋白、β -谱蛋白、α -肌动蛋白、ABP-120、ABP-280和纤蛋白共享同源的肌动蛋白结合结构域，并组成一个肌动蛋白交联蛋白超家族。我们使用退化引物介导的PCR策略确定了这个超家族（ACM）的新成员，该策略经过优化，可以解决不太丰富的超家族序列。ACF7基因位于人类1号染色体上，在northern blots上杂交到高分子量带，序列比较认为ACF7不属于现有家族之一，而是代表了超家族中的一个新类别。
Actin-crosslinking proteins link F-actin into the bundles and networks that constitute the cytoskeleton, Dystrophin, beta-spectrin, alpha-actinin, ABP-120, ABP-280, and fimbrin share homologous actin-binding domains and comprise an actin crosslinker superfamily, We have identified a novel member of this superfamily (ACM) using a degenerate primer-mediated PCR strategy that was optimized to resolve less-abundant superfamily sequences, The ACF7 gene is on human chromosome 1 and hybridizes to high molecular weight bands on northern blots, Sequence comparisons argue that ACF7 does not fit into one of the existing families, but represents a new class within the superfamily,