2 HISTOSPECIFIC ENZYME EXPRESSIONS IN THE SAME CLEAVAGE-ARRESTED ONE-CELLED ASCIDIAN EMBRYOS
2 HISTOSPECIFIC ENZYME EXPRESSIONS IN THE SAME CLEAVAGE-ARRESTED ONE-CELLED ASCIDIAN EMBRYOS
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DOI：
10.1002/jez.1402500208
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发表时间：
1989-05-01
影响因子：
--
通讯作者：
MEEDEL, TH
中科院分区：
文献类型：
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作者：
WHITTAKER, JR;MEEDEL, TH
用细胞松弛素B处理海鞘，玻璃海鞘的受精卵，可以阻止胞质分裂，但不能阻止核分裂。经过适当的时间后，这种分裂逮捕多核受精卵开发的幼虫尾肌乙酰胆碱酯酶和碱性磷酸酶通常位于幼虫内胚层组织。对取自单个动物卵的一对样品中的一个进行单独的组织化学反应提供了实例（6/34），其中显示相应反应产物的细胞松弛素处理的胚胎数量充分重叠（15-29%），表明一些受精卵在同一未裂解的单细胞中发育了两种酶。与acutal双染色技术，可应用于单一的卵裂被捕受精卵，62%的开发一个强大的碱性磷酸酶反应也有很强的乙酰胆碱酯酶反应。在其他实验中，定量测量114个单分裂停滞合子的匀浆中的酶活性直接证实了18%的合子产生两种酶。有没有强制性的相互排斥的潜力，同时表达的两个组织特异性的特点，通常会被隔离到不同的谱系在早期分裂。被认为负责这些组织型表达的细胞质决定簇显然可以在同一细胞中独立发挥作用。
Fertilized eggs of the ascidian, Ciona intestinalis, were prevented from undergoing cytokinesis but not nuclear division by treatment with cytochalasin B. After appropriate times, such cleavage-arrested multinucleate zygotes developed acetylcholinesterase of larval tail muscle and an alkaline phosphatase ordinarily localized in the larval endoderm tissues. Separate histochemical reactions on one of a pair of samples taken from the eggs of single animals provided examples (6/34) in which the numbers of cytochalasin-treated embryos displaying the respective reaction product overlapped sufficiently (15-29%) to indicate that some of the zygotes had developed both enzymes in the same uncleaved single cell. With an acutal dual-staining technique that can be applied to single cleavage-arrested zygotes, 62% of those developing a strong alkaline phosphatase reaction also had a strong acetylcholinesterase reaction. In other experiments, quantitative measurements of enzyme activity in homogenates of 114 single cleavage-arrested zygotes confirm directly that 18% of the zygotes produce both enzymes. There was no obligatory mutual exclusion of the potential for simultaneous expression of two tissue-specific characteristics that would ordinarily be segregated into differnet lineages during early cleavages. The cytoplasmic determinants believed responsbile for these histotypic expressions can apparently function independently in the same cell.