Evidence for human lung stem cells.
Evidence for human lung stem cells.
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DOI：
10.1056/nejmoa1101324
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发表时间：
2011-05-12
期刊：
影响因子：
--
通讯作者：
Anversa P
中科院分区：
文献类型：
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作者：
Kajstura J;Rota M;Hall SR;Hosoda T;D'Amario D;Sanada F;Zheng H;Ogórek B;Rondon-Clavo C;Ferreira-Martins J;Matsuda A;Arranto C;Goichberg P;Giordano G;Haley KJ;Bardelli S;Rayatzadeh H;Liu X;Quaini F;Liao R;Leri A;Perrella MA;Loscalzo J;Anversa P
尽管祖细胞已经在肺的不同解剖区域被描述，但对常驻干细胞的描述仍然难以捉摸。手术肺组织标本原位研究，以识别和表征人肺干细胞。我们定义了它们在体外和体内的表型和功能特性。人肺含有未分化的人肺干细胞，嵌套在远端气道的壁龛中。这些细胞在体外具有自我更新、克隆性和多能性。人肺干细胞在体内注入受损小鼠肺后，形成与受损器官在结构和功能上一体化的人细支气管、肺泡和肺血管。嵌合肺的形成通过检测人上皮和血管基因的转录本得到证实。此外，在连续移植试验中显示了人肺干细胞的自我更新和长期增殖。人类肺部含有可识别的干细胞。在动物模型中，这些细胞参与组织稳态和再生。它们在促进肺部疾病患者的组织修复方面具有尚未证实的潜力。（由美国国立卫生研究院资助。）
Although progenitor cells have been described in distinct anatomical regions of the lung, description of resident stem cells has remained elusive. Surgical lung-tissue specimens were studied in situ to identify and characterize human lung stem cells. We defined their phenotype and functional properties in vitro and in vivo. Human lungs contain undifferentiated human lung stem cells nested in niches in the distal airways. These cells are self-renewing, clonogenic, and multipotent in vitro. After injection into damaged mouse lung in vivo, human lung stem cells form human bronchioles, alveoli, and pulmonary vessels integrated structurally and functionally with the damaged organ. The formation of a chimeric lung was confirmed by detection of human transcripts for epithelial and vascular genes. In addition, the self-renewal and long-term proliferation of human lung stem cells was shown in serial-transplantation assays. Human lungs contain identifiable stem cells. In animal models, these cells participate in tissue homeostasis and regeneration. They have the undemonstrated potential to promote tissue restoration in patients with lung disease. (Funded by the National Institutes of Health.)