IL-27p28 Production by XCR1(+) Dendritic Cells and Monocytes Effectively Predicts Adjuvant-Elicited CD8(+) T Cell Responses.
IL-27p28 Production by XCR1(+) Dendritic Cells and Monocytes Effectively Predicts Adjuvant-Elicited CD8(+) T Cell Responses.
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XCR1(+) 树突状细胞和单核细胞产生的 IL-27p28 可有效预测佐剂引发的 CD8(+) T 细胞反应。
DOI：
10.4049/immunohorizons.1700054
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发表时间：
2018-01-01
期刊：
影响因子：
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通讯作者：
Kedl RM
中科院分区：
文献类型：
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作者：
Kilgore AM;Welsh S;Cheney EE;Chitrakar A;Blain TJ;Kedl BJ;Hunter CA;Pennock ND;Kedl RM
人们普遍认为，先天反应是形成适应性反应的必要先决条件。对于针对感染或辅助亚单位疫苗接种的 T 细胞反应来说也是如此。然而，对于佐剂引发的 T 细胞反应的强度具有预测价值的特定先天参数尚未确定。我们之前报道过亚单位疫苗接种诱导的 T 细胞反应如何依赖于细胞因子 IL-27。这些发现是出乎意料的，因为在没有 IL-27 的情况下，T 细胞对感染的反应通常会增强。使用新型 IL-27p28-eGFP 报告小鼠，我们现在证明佐剂诱导树突状细胞和单核细胞产生 IL-27p28 的程度直接预测所引发的 T 细胞反应的强度。据我们所知，这些数据首次确定了疫苗引发的细胞免疫的具体先天相关性，并且它们对亚单位疫苗生物学具有重要的实践和机制意义。
It is well accepted that the innate response is a necessary prerequisite to the formation of the adaptive response. This is true for T cell responses against infections or adjuvanted subunit vaccination. However, specific innate parameters with predictive value for the magnitude of an adjuvant-elicited T cell response have yet to be identified. We previously reported how T cell responses induced by subunit vaccination were dependent on the cytokine IL-27. These findings were unexpected, given that T cell responses to an infection typically increase in the absence of IL-27. Using a novel IL-27p28–eGFP reporter mouse, we now show that the degree to which an adjuvant induces IL-27p28 production from dendritic cells and monocytes directly predicts the magnitude of the T cell response elicited. To our knowledge, these data are the first to identify a concrete innate correlate of vaccine-elicited cellular immunity, and they have significant practical and mechanistic implications for subunit vaccine biology.