THE PATA GENE-PRODUCT, WHICH CONTAINS A REGION SIMILAR TO CHEY OF ESCHERICHIA-COLI, CONTROLS HETEROCYST PATTERN-FORMATION IN THE CYANOBACTERIUM ANABAENA 7120
THE PATA GENE-PRODUCT, WHICH CONTAINS A REGION SIMILAR TO CHEY OF ESCHERICHIA-COLI, CONTROLS HETEROCYST PATTERN-FORMATION IN THE CYANOBACTERIUM ANABAENA 7120
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DOI：
10.1073/pnas.89.12.5655
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发表时间：
1992-06-15
影响因子：
11.1
通讯作者：
HASELKORN, R
中科院分区：
文献类型：
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作者：
LIANG, J;SCAPPINO, L;HASELKORN, R
在鱼腥藻7120中，异形胞（专门用于固氮的细胞）在细丝的末端和每根细丝内的间隔处发育。我们已经分离出一个突变鱼腥藻菌株，主要是在细丝的末端发育异形胞。该突变体PAT-1在固氮条件下生长不良。对补充PAT-1突变的野生型基因patA进行了克隆和测序。预测的PatA蛋白含有379个氨基酸，分布在三个“域”的基础上预测的亲水性和灵活性。羧基末端结构域与真细菌双组分调节系统中的CheY和其他反应调节剂非常相似。patA突变抑制了由先前描述的野生型hetR基因的额外拷贝产生的多异形胞表型，这表明PatA和HetR是鱼腥藻中相同的环境传感调节回路的组成部分。
In Anabaena 7120, heterocysts (cells specialized for nitrogen fixation) develop at the ends of filaments and at intervals within each filament. We have isolated a mutant Anabaena strain that develops heterocysts mostly at the ends of filaments. This mutant, PAT-1, grows poorly under nitrogen-fixing conditions. The wild-type gene that complements the mutation in PAT-1, called patA, was cloned and sequenced. The predicted PatA protein contains 379 amino acids distributed among three "domains" based on predictions of hydropathy and flexibility. The carboxyl-terminal domain is very similar to that of CheY and other response regulators in two-component regulatory systems in eubacteria. The patA mutation suppresses the multiheterocyst phenotype produced by extra copies of the wild-type hetR gene described previously, suggesting that PatA and HetR are components of the same environment-sensing regulatory circuit in Anabaena.